Department of Biomedical Engineering, 107 W. Dean Keeton St., C0800, Austin, TX, 78712, USA.
Department of Biomedical Engineering, 107 W. Dean Keeton St., C0800, Austin, TX, 78712, USA; Institute for Cellular and Molecular Biology, The University of Texas at Austin, Norman Hackerman Building, 100 East 24th St., NHB 4500, Austin, TX, 78712, USA.
Curr Opin Cell Biol. 2020 Aug;65:72-77. doi: 10.1016/j.ceb.2020.02.017. Epub 2020 Mar 28.
Intracellular trafficking requires extensive changes in membrane morphology. Cells use several distinct molecular factors and physical cues to remodel membranes. Here, we highlight recent advances in identifying the biophysical mechanisms of membrane curvature generation. In particular, we focus on the cooperation of molecular and physical drivers of membrane bending during three stages of vesiculation: budding, cargo selection, and scission. Taken together, the studies reviewed here emphasize that, rather than a single dominant mechanism, several mechanisms typically work in parallel during each step of membrane remodeling. Important challenges for the future of this field are to understand how multiple mechanisms work together synergistically and how a series of stochastic events can be combined to achieve a deterministic result-assembly of the trafficking vesicle.
细胞内运输需要膜形态的广泛改变。细胞使用几种不同的分子因子和物理线索来重塑膜。在这里，我们重点介绍了识别膜曲率产生的生物物理机制的最新进展。特别是，我们专注于分子和物理驱动因素在囊泡形成的三个阶段（出芽、货物选择和分裂）期间对膜弯曲的合作。综上所述，这里回顾的研究强调，在膜重塑的每个步骤中，通常不是单一的主导机制，而是几种机制通常协同工作。该领域未来的重要挑战是了解多种机制如何协同工作，以及一系列随机事件如何组合以实现确定性结果——运输囊泡的组装。
