Chen Mengdan, Wang Ying, Chen Jiajia, Cao Jinjun
Department of Plant Protection, Henan Institute of Science and Technology, Xinxiang, China.
Mitochondrial DNA B Resour. 2019 Oct 7;4(2):3392-3393. doi: 10.1080/23802359.2019.1674723.
One new mitochondrial genome of from the family Nemouridae (Insecta: Plecoptera) was sequenced in the study. The mitochondrial genome has the length of 15,718 bp, encoding 37 genes: 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, and 2 ribosomal RNA (rRNA) genes. The whole nucleotide composition biased adenine and thymine with A + T accounting for 69.9%. Nine PCGs and 14 tRNA genes are encoded in the J chain, the other four PCGs, eight tRNAs, and two rRNA genes are encoded in the chain of N. The mitochondrial genome includes 13 gene overlaps and 12 intergenic spacers. Most PCGs strictly use the ATN as start codon, and terminate with traditional stop codon (TAA and TAG). Except the seems to lack dihydrogen glycine arm, all tRNA genes of the mitochondrial genome are the typical clover secondary structure. The phylogenetic tree of PCGs dataset based on bayesian inference (BI) and maximum likelihood (ML) analysis show the same tree topology. Both ML and BI analysis support the sister-group relationship between and . Meanwhile, Capniidae is closely related to Taeniopterygidae.
在该研究中对来自叉襀科（昆虫纲：襀翅目）的一个新线粒体基因组进行了测序。该线粒体基因组长度为15,718 bp，编码37个基因：13个蛋白质编码基因（PCGs）、22个转移RNA（tRNA）基因和2个核糖体RNA（rRNA）基因。整个核苷酸组成偏向腺嘌呤和胸腺嘧啶，A+T占69.9%。9个PCGs和14个tRNA基因由J链编码，其他4个PCGs、8个tRNAs和2个rRNA基因由N链编码。线粒体基因组包括13个基因重叠区和12个基因间隔区。大多数PCGs严格使用ATN作为起始密码子，并以传统终止密码子（TAA和TAG）终止。除了似乎缺乏二氢甘氨酸臂外，线粒体基因组的所有tRNA基因均为典型的三叶草二级结构。基于贝叶斯推断（BI）和最大似然（ML）分析的PCGs数据集的系统发育树显示出相同的树形拓扑结构。ML和BI分析均支持与之间的姐妹群关系。同时，小石蝇科与长角石蝇科密切相关。
