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CeO纳米颗粒的干式生成及其在气液界面上沉积到A549和THP-1细胞共培养物上——剂量学考量及与浸没暴露的比较

Dry Generation of CeO Nanoparticles and Deposition onto a Co-Culture of A549 and THP-1 Cells in Air-Liquid Interface-Dosimetry Considerations and Comparison to Submerged Exposure.

作者信息

Cappellini Francesca, Di Bucchianico Sebastiano, Karri Venkatanaidu, Latvala Siiri, Malmlöf Maria, Kippler Maria, Elihn Karine, Hedberg Jonas, Odnevall Wallinder Inger, Gerde Per, Karlsson Hanna L

机构信息

Institute of Environmental Medicine, Karolinska Institutet, Stockholm, 17177 Sweden.

Comprehensive Molecular Analytics, Helmholtz Zentrum München, 81379 München, Germany.

出版信息

Nanomaterials (Basel). 2020 Mar 27;10(4):618. doi: 10.3390/nano10040618.

DOI:10.3390/nano10040618
PMID:32230801
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7221976/
Abstract

Relevant in vitro assays that can simulate exposure to nanoparticles (NPs) via inhalation are urgently needed. Presently, the most common method employed is to expose lung cells under submerged conditions, but the cellular responses to NPs under such conditions might differ from those observed at the more physiological air-liquid interface (ALI). The aim of this study was to investigate the cytotoxic and inflammatory potential of CeO NPs (NM-212) in a co-culture of A549 lung epithelial cells and differentiated THP-1 cells in both ALI and submerged conditions. Cellular dose was examined quantitatively using inductively coupled plasma mass spectrometry (ICP-MS). The role of serum and LPS-priming for IL-1β release was further tested in THP-1 cells in submerged exposure. An aerosol of CeO NPs was generated by using the PreciseInhale system, and NPs were deposited on the co-culture using Xpose. No or minor cytotoxicity and no increased release of inflammatory cytokines (IL-1β, IL-6, TNFα, MCP-1) were observed after exposure of the co-culture in ALI (max 5 µg/cm) or submerged (max 22 µg/cm) conditions. In contrast, CeO NPs cause clear IL-1β release in monocultures of macrophage-like THP-1, independent of the presence of serum and LPS-priming. This study demonstrates a useful approach for comparing effects at various in-vitro conditions.

摘要

迫切需要能够模拟通过吸入接触纳米颗粒（NP）的相关体外试验。目前，最常用的方法是在浸没条件下使肺细胞暴露，但在这种条件下细胞对NP的反应可能与在更接近生理状态的气液界面（ALI）观察到的反应不同。本研究的目的是在ALI和浸没条件下，研究CeO NP（NM-212）在A549肺上皮细胞和分化的THP-1细胞共培养物中的细胞毒性和炎症潜力。使用电感耦合等离子体质谱（ICP-MS）对细胞剂量进行定量检测。在浸没暴露的THP-1细胞中进一步测试了血清和LPS引发对IL-1β释放的作用。使用PreciseInhale系统产生CeO NP气溶胶，并使用Xpose将NP沉积在共培养物上。在ALI（最大5μg/cm）或浸没（最大22μg/cm）条件下暴露共培养物后，未观察到细胞毒性或轻微细胞毒性，也未观察到炎性细胞因子（IL-1β、IL-6、TNFα、MCP-1）释放增加。相比之下，CeO NP在巨噬细胞样THP-1的单培养物中导致明显的IL-1β释放，与血清和LPS引发的存在无关。本研究展示了一种在各种体外条件下比较效应的有用方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cf/7221976/45417307d739/nanomaterials-10-00618-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cf/7221976/7aff6f681b99/nanomaterials-10-00618-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cf/7221976/a0a5724bbb40/nanomaterials-10-00618-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cf/7221976/17f734e9ad9c/nanomaterials-10-00618-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cf/7221976/0c17577dac45/nanomaterials-10-00618-g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cf/7221976/45417307d739/nanomaterials-10-00618-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cf/7221976/7aff6f681b99/nanomaterials-10-00618-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cf/7221976/a0a5724bbb40/nanomaterials-10-00618-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cf/7221976/17f734e9ad9c/nanomaterials-10-00618-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cf/7221976/0c17577dac45/nanomaterials-10-00618-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cf/7221976/0d25c59893d5/nanomaterials-10-00618-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cf/7221976/45417307d739/nanomaterials-10-00618-g006.jpg

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