Pickett Brian R, Gulzar Asim, Ferré Juan, Wright Denis J
Department of Life Sciences, Imperial College of Science, Technology and Medicine, London, Silwood Park Campus, Ascot, Berkshire, United Kingdom.
Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire, United Kingdom.
Appl Environ Microbiol. 2017 Apr 17;83(9). doi: 10.1128/AEM.03506-16. Print 2017 May 1.
Laboratory selection with Vip3Aa of a field-derived population of produced >2,040-fold resistance in 12 generations of selection. The Vip3Aa-selected (Vip-Sel)-resistant population showed little cross-resistance to Cry1Ab and no cross-resistance to Cry1Ac. Resistance was unstable after 15 generations without exposure to the toxin. F reciprocal crosses between Vip3Aa-unselected (Vip-Unsel) and Vip-Sel insects indicated a strong paternal influence on the inheritance of resistance. Resistance ranged from almost completely recessive (mean degree of dominance [] = 0.04 if the resistant parent was female) to incompletely dominant (mean = 0.53 if the resistant parent was male). Results from bioassays on the offspring from backcrosses of the F progeny with Vip-Sel insects indicated that resistance was due to more than one locus. The results described in this article provide useful information for the insecticide resistance management strategies designed to overcome the evolution of resistance to Vip3Aa in insect pests. is an important pest that has the ability to feed on many plant species. The extensive use of (Bt) crops or spray has already led to the evolution of insect resistance in the field for some species of Lepidoptera and Coleoptera. The development of resistance in insect pests is the main threat to Bt crops. The effective resistance management strategies are very important to prolong the life of Bt plants. Lab selection is the key step to test the assumption and predictions of management strategies prior to field evaluation. Resistant insects offer useful information to determine the inheritance of resistance and the frequency of resistance alleles and to study the mechanism of resistance to insecticides.
用Vip3Aa对田间种群进行实验室选育，在12代选育中产生了>2040倍的抗性。经Vip3Aa选育（Vip-Sel）的抗性种群对Cry1Ab几乎没有交叉抗性，对Cry1Ac也没有交叉抗性。在未接触毒素的15代后，抗性变得不稳定。Vip3Aa未选育（Vip-Unsel）昆虫与Vip-Sel昆虫之间的正反交表明，父本对抗性遗传有强烈影响。抗性范围从几乎完全隐性（如果抗性亲本为雌性，平均显性度[] = 0.04）到不完全显性（如果抗性亲本为雄性，平均显性度 = 0.53）。对F代与Vip-Sel昆虫回交后代的生物测定结果表明，抗性是由多个基因座引起的。本文所述结果为旨在克服害虫对Vip3Aa抗性进化的杀虫剂抗性管理策略提供了有用信息。是一种重要害虫，能够取食多种植物物种。广泛使用（Bt）作物或喷雾已经导致一些鳞翅目和鞘翅目物种在田间产生了昆虫抗性。害虫抗性的发展是Bt作物的主要威胁。有效的抗性管理策略对于延长Bt植物的寿命非常重要。实验室选育是在田间评估之前测试管理策略假设和预测的关键步骤。抗性昆虫为确定抗性遗传、抗性等位基因频率以及研究杀虫剂抗性机制提供了有用信息。
