Schmitt R E, Haas J, Amadò R
Institut für Lebensmittelwissenschaft, ETH Zentrum, Zürich, Schweiz.
Z Lebensm Unters Forsch. 1988 Aug;187(2):121-4. doi: 10.1007/BF01042622.
A simple high-performance liquid chromatographic (HPLC) analysis is described for the determination of biogenic amines in broiler carcasses. The clean-up procedure consists of an extraction with 0.6 M-perchloric acid, formation of dansyl derivatives, separation by a RP-18 column and UV detection at 254 nm. Within 7 min eight amines could be separated. The quantitative determination of spermidine and spermine requires an additional ion-exchange clean-up with Amberlite CG 50 after the extraction. This procedure gives recoveries of 82%-96% with detection limits of 0.2-0.5 microgram/g of broiler skin. Putrescine and cadaverine are good indicators for the onset of spoilage of poultry carcasses, since both amines could be detected at total colony counts of 10(5) cfu/cm2 and their concentration increases rapidly with advancing decomposition.
描述了一种用于测定肉鸡胴体中生物胺的简单高效液相色谱（HPLC）分析方法。净化程序包括用0.6M高氯酸萃取、丹磺酰衍生物的形成、通过RP - 18柱分离以及在254nm处进行紫外检测。在7分钟内可分离出8种胺。精胺和亚精胺的定量测定在萃取后需要用Amberlite CG 50进行额外的离子交换净化。该方法的回收率为82% - 96%，检测限为0.2 - 0.5微克/克肉鸡皮肤。腐胺和尸胺是家禽胴体腐败开始的良好指标，因为在总菌落数为10(5) cfu/cm2时就能检测到这两种胺，并且随着分解的推进它们的浓度迅速增加。
