Paleologos E K, Chytiri S D, Savvaidis I N, Kontominas M G
University of Ioannina, Department of Chemistry, Laboratory of Food Chemistry and Technology, 45110 Ioannina, Greece.
J Chromatogr A. 2003 Aug 29;1010(2):217-24. doi: 10.1016/s0021-9673(03)01068-9.
The advantages of micellar cloud point extraction combined with a surfactant-assisted separation in a HPLC system are presented as a method for the effective separation and determination of nine biogenic amines in fish substrates. Benzoyl derivatives of the amines are extracted inside the micelles of a non-ionic surfactant, Triton X-114, and separated with gradient elution micellar liquid chromatography. Quantification was performed by measuring the UV absorbance of the benzene ring at 254 nm. Detection limits of the nine biogenic amines were in the vicinity of 0.01 mg l(-1) which are approximately 10 times lower than those of the conventional method (HPLC-UV) and 100 times lower than those of micellar electrokinetic capillary chromatography. The correlation coefficients of determinations were 0.9911-0.9996. The method was applied for the determination of putrescine, cadaverine, agmatine, tyramine, tryptamine, phenylethylamine, spermine, spermidine and histamine in trout samples. Recovery of the proposed method ranged from 95 to 103.5%.
本文介绍了胶束相浊点萃取结合高效液相色谱系统中的表面活性剂辅助分离法，作为一种有效分离和测定鱼类基质中9种生物胺的方法。胺的苯甲酰衍生物在非离子表面活性剂Triton X - 114的胶束中被萃取，并通过梯度洗脱胶束液相色谱法进行分离。通过测量254 nm处苯环的紫外吸光度进行定量。9种生物胺的检测限在0.01 mg l(-1)左右，约为传统方法（高效液相色谱 - 紫外检测法）的1/10，胶束电动毛细管色谱法的1/100。测定的相关系数为0.9911 - 0.9996。该方法用于测定鳟鱼样品中的腐胺、尸胺、胍丁胺、酪胺、色胺、苯乙胺、精胺、亚精胺和组胺。该方法的回收率在95%至103.5%之间。
