Subramaniyan Sivakumar Allur, Kang Darae, Siddiqui Sharif Hasan, Park Jinryong, Tian Weishun, Park Byungyong, Shim Kwanseob
Department of Animal Biotechnology, College of Agriculture and Life Sciences, Jeonbuk National University, Jeonju 54896, Korea.
College of Veterinary Medicine and Bio-Safety Research Institute, Jeonbuk National University, Iksan 54596, Korea.
Animals (Basel). 2020 Mar 27;10(4):564. doi: 10.3390/ani10040564.
The aim of the study was to analyze the injection of inorganic and organic synthesized silver nanoparticles (Ag NPs) using L. var. capitate . (BOL) conjugation with L-Arginine (L-Arg) on the immune, muscle growth, survivability and hatchability of broiler chickens. The conjugation of L-Arg (100 μg) with 1000 µg of Ag NPs synthesized by (BOL)-extract and L-Arg (100 μg) conjugated with 100 µg of Ag NPs inorganic synthesized were injected into fertile eggs at 8 d, 14 d and 18 d of incubation. Survival and hatching rate were significantly improved in the dose of L-Arg (100 μg) with 1000 µg (BOL-Ag NPs) and L-Arg (100 μg) with 100 µg (C-Ag NPs) on 14 d injection whereas it was decreased on 8 d or 18 d injection. Moreover, the protein expression of muscle development markers such as myogenin and myoD were significantly uprelated in 14 d of incubation whereas the heat shock proteins (HSPs), such as HSP-60 and HSP-70, were significantly upregulated in 18 d incubation. In addition, the liver function marker of serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) were significantly decreased and the immunoglobulin (IgM) levels were increased in a 14 d incubation period in serum at the same concentration.
本研究的目的是分析使用头状罗勒（BOL）与L-精氨酸（L-Arg）结合的无机和有机合成银纳米颗粒（Ag NPs）对肉鸡免疫、肌肉生长、存活率和孵化率的影响。将100 μg L-Arg与由（BOL）提取物合成的1000 μg Ag NPs结合物以及100 μg L-Arg与100 μg无机合成Ag NPs结合物在孵化的第8天、第14天和第18天注射到受精蛋中。在第14天注射时，100 μg L-Arg与1000 μg（BOL-Ag NPs）以及100 μg L-Arg与100 μg（C-Ag NPs）剂量组的存活率和孵化率显著提高，而在第8天或第18天注射时则降低。此外，在孵化第14天时，肌肉发育标志物如肌细胞生成素和肌分化抗原（MyoD）的蛋白表达显著上调，而在孵化第18天时，热休克蛋白（HSPs）如HSP-60和HSP-70显著上调。此外，在相同浓度下，孵化第14天时血清谷丙转氨酶（SGOT）和血清谷草转氨酶（SGPT）这两种肝功能标志物显著降低，血清免疫球蛋白（IgM）水平升高。
