• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

使用十二烷基硫酸钠-凝胶电泳法测定鸡蛋及蛋制品中的溶菌酶含量。

Determination of lysozyme content in eggs and egg products using SDS-gel electrophoresis.

作者信息

Weth F, Schroeder T, Buxtorf U P

机构信息

Kantonales Laboratorium Basel-Stadt, Switzerland.

出版信息

Z Lebensm Unters Forsch. 1988 Dec;187(6):541-5. doi: 10.1007/BF01042386.

DOI:10.1007/BF01042386
PMID:3223094
Abstract

Lysozyme can be separated commercially from other egg components and is used as a natural, antibiotically active protein. A method for the detection of de-lysozymed egg products using SDS-polyacrylamide gel electrophoresis (PAGE) is described. The method can also be used for lysozyme quantification. Lysozyme concentrations in egg products are tentatively assigned to three classes, based on the lysozyme contents found in intact eggs. The results obtained from a series of egg products on the market allow two groups to be distinguished. The first group has values for average lysozyme content, standard deviation and total protein content very close to the values found for intact eggs, and the second group clearly shows a lower lysozyme content but a total protein content comparable with intact eggs.

摘要

溶菌酶可从鸡蛋的其他成分中进行商业分离,并用作一种具有天然抗菌活性的蛋白质。本文描述了一种使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)检测去溶菌酶鸡蛋产品的方法。该方法也可用于溶菌酶的定量分析。根据完整鸡蛋中的溶菌酶含量,将蛋制品中的溶菌酶浓度初步分为三类。从市场上一系列蛋制品获得的结果可以区分出两组。第一组的平均溶菌酶含量、标准差和总蛋白含量的值与完整鸡蛋中的值非常接近,第二组则明显显示出较低的溶菌酶含量,但总蛋白含量与完整鸡蛋相当。

相似文献

1
Determination of lysozyme content in eggs and egg products using SDS-gel electrophoresis.使用十二烷基硫酸钠-凝胶电泳法测定鸡蛋及蛋制品中的溶菌酶含量。
Z Lebensm Unters Forsch. 1988 Dec;187(6):541-5. doi: 10.1007/BF01042386.
2
Development of an ELISA for quantifying lysozyme in hen egg white.一种用于定量测定蛋清中溶菌酶的酶联免疫吸附测定法的开发。
J Agric Food Chem. 2005 Apr 6;53(7):2379-85. doi: 10.1021/jf048692o.
3
Characterization of four major allergens of hen egg-white by IEF/SDS-PAGE combined with electrophoretic transfer and IgE-immunoautoradiography.通过IEF/SDS-PAGE结合电泳转移和IgE免疫放射自显影法对蛋清的四种主要过敏原进行表征。
Int Arch Allergy Appl Immunol. 1990;91(2):136-41. doi: 10.1159/000235104.
4
Heterogeneity of serum, egg amylase and egg lysozyme in the interspecific hybrids of ducks.
Comp Biochem Physiol B. 1983;75(2):323-6. doi: 10.1016/0305-0491(83)90333-4.
5
Determination of egg white lysozyme by on-line coupled capillary isotachophoresis with capillary zone electrophoresis.在线耦合毛细管等速电泳与毛细管区带电泳联用测定蛋清溶菌酶
Electrophoresis. 2003 Mar;24(5):860-4. doi: 10.1002/elps.200390108.
6
Comparative antibacterial activity of avian egg white protein extracts.禽蛋清蛋白提取物的抗菌活性比较
Br Poult Sci. 2008 Mar;49(2):125-32. doi: 10.1080/00071660801938825.
7
Qualification and quantification of fish protein in prepared surimi crabstick.制备的鱼糜蟹棒中鱼蛋白的鉴定与定量分析。
J Food Sci. 2008 Jun;73(5):C329-34. doi: 10.1111/j.1750-3841.2008.00759.x.
8
Changes in egg white during incubation of the fertile egg.
Poult Sci. 1974 Jul;53(4):1561-5. doi: 10.3382/ps.0531561.
9
Effect of polymorphism in egg white lysozyme on muramidase and antibacterial activities as well as hatchability in the Japanese quail (Coturnix japonica).蛋清溶菌酶多态性对日本鹌鹑(Coturnix japonica)的溶菌酶和抗菌活性以及孵化率的影响。
J Anim Sci. 2012 Jun;90(6):1747-55. doi: 10.2527/jas.2011-4501. Epub 2011 Dec 28.
10
Sequential separation of lysozyme, ovomucin, ovotransferrin, and ovalbumin from egg white.从蛋清中顺序分离溶菌酶、卵黏蛋白、卵转铁蛋白和卵白蛋白。
Poult Sci. 2014 Apr;93(4):1001-9. doi: 10.3382/ps.2013-03403.

引用本文的文献

1
Study of Lysozyme Activity in Bird Egg Whites by Fluorescence Polarization Assay Using Chitooligosaccharide Tracer.使用壳寡糖示踪剂通过荧光偏振分析法研究禽蛋蛋清中的溶菌酶活性。
Foods. 2025 Apr 15;14(8):1365. doi: 10.3390/foods14081365.
2
Antibacterial Properties of TMA against and Effect of Temperature and Storage Duration on TMA Content, Lysozyme Activity and Content in Eggs.三甲胺对鸡蛋的抗菌特性以及温度和储存时间对鸡蛋中三甲胺含量、溶菌酶活性和含量的影响。
Foods. 2022 Feb 11;11(4):527. doi: 10.3390/foods11040527.
3
Molecularly Imprinted Polyscopoletin for the Electrochemical Detection of the Chronic Disease Marker Lysozyme.
基于分子印迹聚多糖的电化学检测慢性疾病标志物溶菌酶
Biosensors (Basel). 2020 Dec 23;11(1):3. doi: 10.3390/bios11010003.
4
Lysozyme aptasensor based on a glassy carbon electrode modified with a nanocomposite consisting of multi-walled carbon nanotubes, poly(diallyl dimethyl ammonium chloride) and carbon quantum dots.基于玻碳电极修饰的纳米复合材料的溶菌酶适体传感器,该纳米复合材料由多壁碳纳米管、聚二烯丙基二甲基氯化铵和碳量子点组成。
Mikrochim Acta. 2018 Feb 14;185(3):180. doi: 10.1007/s00604-017-2656-7.
5
Different analytical approaches in assessing antibacterial activity and the purity of commercial lysozyme preparations for dairy application.评估抗菌活性和商业溶菌酶制剂用于乳品应用的纯度的不同分析方法。
Molecules. 2013 May 21;18(5):6008-20. doi: 10.3390/molecules18056008.
6
Competition-mediated pyrene-switching aptasensor: probing lysozyme in human serum with a monomer-excimer fluorescence switch.竞争介导的芘开关适体传感器:利用单体-二聚体荧光开关检测人血清中的溶菌酶。
Anal Chem. 2010 Dec 15;82(24):10158-63. doi: 10.1021/ac102277p. Epub 2010 Nov 16.