Takenaka H, Kusumi T, Mizukoshi O
Department of Otorhinolaryngology, Kyoto Prefectural University of Medicine, Japan.
Acta Otolaryngol Suppl. 1988;454:133-7. doi: 10.3109/00016488809125016.
Using anti-human IgE monoclonal antibody E235I63, an IgE antibody to mite extract response by peripheral blood lymphocytes (PBL) and tonsillar lymphoid cells from 3 atopic patients suffering nasal allergy and 2 normal individuals was detected by a sensitive radioimmunoassay of culture supernatants. Although the spontaneous IgE antibody response was characteristic of atopic PBL, PBL of normal controls did not produce anti-mite IgE antibody spontaneously. IgE antibody to mite extracts were detected in supernatants of all tonsillar cells cultured with mite antigen or PWM, but PBL from only atopic donors could produce IgE antibody in the same conditions.
利用抗人IgE单克隆抗体E235I63，通过对培养上清液进行灵敏的放射免疫测定，检测了3名患有鼻过敏的特应性患者和2名正常个体的外周血淋巴细胞（PBL）及扁桃体淋巴样细胞对螨提取物的IgE抗体反应。虽然自发性IgE抗体反应是特应性PBL的特征，但正常对照的PBL不会自发产生抗螨IgE抗体。在用螨抗原或PWM培养的所有扁桃体细胞的上清液中均检测到了针对螨提取物的IgE抗体，但只有来自特应性供体的PBL能在相同条件下产生IgE抗体。
