Harabuchi Y, Hamamoto M, Shirasaki H, Asakura K, Matsuyama H, Kataura A
Department of Otolaryngology, Sapporo Medical College, Japan.
Am J Otolaryngol. 1989 Mar-Apr;10(2):138-42. doi: 10.1016/0196-0709(89)90137-3.
The specific antibody response of the adenoids to a respiratory antigen was investigated, both in vitro and in vivo. The in vitro immunoglobulin (Ig) production of adenoidal and tonsillar lymphocytes in the same patient in 18 cases was measured by an enzyme-linked immunosorbent assay. Adenoidal lymphocytes produced more IgM than IgG or IgA under culture conditions without any mitogens, whereas IgG was the major Ig produced by tonsillar lymphocytes. The same results were obtained when cultured with pokeweed mitogen. Under culture conditions with Dermatophagoides farinae (mite) antigen, adenoidal lymphocytes produced only specific IgM class antibody, and at a level significantly greater than tonsillar lymphocytes. Through an in vivo study, we established experimental adenoidal tissue in the guinea pig by long-term exposure to an ovalbumin aerosol. Infiltration of cells producing the specific antibody against an ovalbumin was demonstrated in the epipharyngeal mucosa using immunofluorescent staining. Our results confirm that the adenoids play a role in specific immune responses to respiratory antigens.
对腺样体针对呼吸道抗原的特异性抗体反应进行了体内和体外研究。采用酶联免疫吸附测定法检测了18例患者腺样体和扁桃体淋巴细胞的体外免疫球蛋白（Ig）产生情况。在无任何促有丝分裂原的培养条件下，腺样体淋巴细胞产生的IgM多于IgG或IgA，而扁桃体淋巴细胞产生的主要Ig是IgG。用商陆有丝分裂原培养时也得到了相同结果。在粉尘螨抗原培养条件下，腺样体淋巴细胞仅产生特异性IgM类抗体，且水平显著高于扁桃体淋巴细胞。通过体内研究，我们通过长期暴露于卵清蛋白气雾剂在豚鼠体内建立了实验性腺样体组织。采用免疫荧光染色在咽上黏膜中证实了产生抗卵清蛋白特异性抗体的细胞浸润。我们的结果证实腺样体在针对呼吸道抗原的特异性免疫反应中发挥作用。
