Biology Centre of the Czech Academy of Sciences, Institute of Plant Molecular Biology, Branišovská 31, 37005, České Budějovice, Czech Republic.
Arch Virol. 2020 May;165(5):1249-1252. doi: 10.1007/s00705-020-04603-9. Epub 2020 Mar 30.
In this study, the complete genomic sequence of a novel virus was determined by next-generation sequencing of a sample from a symptomatic strawberry plant with severe yellow spots and mosaic on its leaves. Its genomic organization and sequence showed that this virus is related to members of the proposed insect-specific genus "Negevirus". The sample also contained sequences from the geranium aphid Acyrthosiphon malvae. Although the virus was detected repeatedly in the same plant during the three following years, no other positive samples were obtained from the surroundings or more-distant locations. Reverse transcription qPCR analysis revealed the presence of both genomic positive and complementary negative strands of the viral genome in the sample, with a 3- to 30-fold excess of the positive strand, indicating active viral replication. As the virus was not detected in any insect species collected at this location, the virus was provisionally named "Fragaria vesca-associated virus 1" (FVaV-1).
在这项研究中，通过对一株有严重黄点和花叶症状的草莓植株样本进行下一代测序，确定了一种新型病毒的完整基因组序列。其基因组结构和序列表明，该病毒与拟议的昆虫特异性属“Negevirus”的成员有关。该样本还包含来自天竺葵蚜虫 Acyrthosiphon malvae 的序列。尽管在随后的三年中，该病毒在同一株植物中反复被检测到，但在周围或更远的地方没有获得其他阳性样本。反转录 qPCR 分析显示，该样本中存在病毒基因组的正链和互补负链，正链的数量是负链的 3 到 30 倍，表明病毒复制活跃。由于在该地点收集的任何昆虫物种中都未检测到该病毒，因此该病毒被暂时命名为“草莓 associatedvirus1”（FVaV-1）。
