State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Viruses. 2020 Jun 27;12(7):692. doi: 10.3390/v12070692.
Negeviruses are a proposed group of insect-specific viruses that can be separated into two distinct phylogenetic clades, Nelorpivirus and Sandewavirus. Negeviruses are well-known for their wide geographic distribution and broad host range among hematophagous insects. In this study, the full genomes of two novel negeviruses from each of these clades were identified by RNA extraction and sequencing from a single dungfly () collected from the Arctic Yellow River Station, where these genomes are the first negeviruses from cold zone regions to be discovered. Nelorpivirus dungfly1 (NVD1) and Sandewavirus dungfly1 (SVD1) have the typical negevirus genome organization and there was a very high coverage of viral transcripts. Small interfering RNAs derived from both viruses were readily detected in , clearly showing that negeviruses are targeted by the host antiviral RNA interference (RNAi) pathway. These results and subsequent analysis (studies) of public database and published virome data showed that the hosts of nege-like viruses include insects belonging to many orders as well as various non-insects in addition to the hematophagous insects previously reported. Phylogenetic analysis reveals at least three further groups of negeviruses, as well as several poorly resolved solitary branches, filling in the gaps within the two sub-groups of negeviruses and plant-associated viruses in the . The results of this study will contribute to a better understanding of the geographic distribution, host range, evolution and host antiviral immune responses of negeviruses.
尼格病毒是一类被提议的昆虫特异性病毒，可以分为两个不同的系统发育分支，Nelorpivirus 和 Sandewavirus。尼格病毒以广泛的地理分布和在吸血昆虫中的广泛宿主范围而闻名。在这项研究中，通过从从北极黄河站采集的一只蜉蝣中提取和测序 RNA，鉴定了来自这两个分支的两种新型尼格病毒的全基因组。Nelorpivirus 蜉蝣 1（NVD1）和 Sandewavirus 蜉蝣 1（SVD1）具有典型的尼格病毒基因组结构，并且病毒转录本的覆盖率非常高。两种病毒的小干扰 RNA 都可以在 中被轻易检测到，这清楚地表明尼格病毒是宿主抗病毒 RNA 干扰（RNAi）途径的靶标。这些结果以及随后对公共数据库和已发表病毒组数据的 分析（研究）表明，尼格样病毒的宿主包括许多目昆虫以及以前报道的吸血昆虫以外的各种非昆虫。系统发育分析显示，至少还有三组新的尼格病毒，以及几个分辨率较低的孤立分支，填补了 Nelorpivirus 和 Sandewavirus 两个亚群以及 中与植物相关病毒之间的空白。这项研究的结果将有助于更好地了解尼格病毒的地理分布、宿主范围、进化和宿主抗病毒免疫反应。
