森林草莓（Fragaria vesca）对草莓脉带病毒（SVBV）感染反应的转录组分析

Transcriptome analysis of woodland strawberry (Fragaria vesca) response to the infection by Strawberry vein banding virus (SVBV).

作者信息

Chen Jing, Zhang Hanping, Feng Mingfeng, Zuo Dengpan, Hu Yahui, Jiang Tong

机构信息

School of Plant Protection, Anhui Agricultural University, Hefei, 230036, People's Republic of China.

出版信息

Virol J. 2016 Jul 13;13:128. doi: 10.1186/s12985-016-0584-5.

BACKGROUND

Woodland strawberry (Fragaria vesca) infected with Strawberry vein banding virus (SVBV) exhibits chlorotic symptoms along the leaf veins. However, little is known about the molecular mechanism of strawberry disease caused by SVBV.

METHODS

We performed the next-generation sequencing (RNA-Seq) study to identify gene expression changes induced by SVBV in woodland strawberry using mock-inoculated plants as a control.

RESULTS

Using RNA-Seq, we have identified 36,850 unigenes, of which 517 were differentially expressed in the virus-infected plants (DEGs). The unigenes were annotated and classified with Gene Ontology (GO), Clusters of Orthologous Group (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The KEGG pathway analysis of these genes suggested that strawberry disease caused by SVBV may affect multiple processes including pigment metabolism, photosynthesis and plant-pathogen interactions.

CONCLUSIONS

Our research provides comprehensive transcriptome information regarding SVBV infection in strawberry.

背景

感染草莓脉带病毒（SVBV）的森林草莓（Fragaria vesca）沿叶脉出现褪绿症状。然而，关于SVBV引起草莓病害的分子机制知之甚少。

方法

我们进行了新一代测序（RNA测序）研究，以未接种的植株作为对照，鉴定SVBV在森林草莓中诱导的基因表达变化。

结果

通过RNA测序，我们鉴定出36,850个单基因，其中517个在病毒感染植株中差异表达（差异表达基因）。这些单基因通过基因本体论（GO）、直系同源簇（COG）和京都基因与基因组百科全书（KEGG）分析进行注释和分类。对这些基因的KEGG通路分析表明，SVBV引起的草莓病害可能影响多个过程，包括色素代谢、光合作用和植物-病原体相互作用。