Ma Tie-Liang, Tan Zhi-An, Qiao Bin, Lu Sheng-Jie, Zhu Wen-Jiao
Department of Andrology, Yixing People's Hospital, Yixing, Jiangsu 214200, China.
Zhonghua Nan Ke Xue. 2019 Sep;25(9):828-832.
To investigate the effect and action mechanism of Yu Si Granules (YSG) in the treatment methyl methanesulphonate (MMS)-induced oligoasthenozoospermia (OAZ) in mice.
Thirty adult male mice were randomly divided into three groups of equal number, normal control, OAZ model control and YSG intervention. The OAZ model was established by oral administration of MMS and the model mice in the YSG intervention group were treated intragastrically with YSG suspension at 0.144 g/100 g of the body weight per day for 48 successive days. Then, all the mice were sacrificed and their epididymides harvested for detection of the sperm count and motility, observation of the morphology of the seminiferous tubules by HE staining, determination of the expressions of the germ cell-, sperm cell-, spermatocyte-, Sertoli cell- and blood-testis barrier-related genes by RT-PCR, and measurement of the levels of oxidative stress in the blood.
Compared with the normal control, the OAZ model mice showed significantly decreased sperm count (［49.2 ± 0.7］ vs ［23.6 ± 0.4］ ×107/ml/g, P < 0.05) and sperm motility (［76.3 ± 0.7］% vs ［5.0 ± 5.8］%, P < 0.05), which were both remarkably increased after YSG intervention (［38.4 ± 0.5］ ×107/ml/g and ［71.5 ± 0.5］%) (P < 0.05). The OAZ model mice also exhibited degenerated and atrophic seminiferous tubules, thinner seminiferous epithelia, disorderly arranged cells at different levels, reduced number of sperm in the lumen and unclear layers of germ cells in the epididymis, while those after YSG intervention manifested regularly organized seminiferous tubules with orderly arrangement and clear layers. The expressions of the Vasa, Dazl and Snd1 genes were significantly decreased (P < 0.05), but not those of Gfra, Plzf, Stra8, Spo11, Sycp3, Sox9 and Vim (P > 0.05) in the OAZ model and YSG intervention groups as compared with those in the normal control group. The superoxide dismutase (SOD) activity in the serum was markedly reduced in the OAZ model mice as compared with that in the normal controls (P < 0.05) and increased again after YSP intervention (P < 0.05), but the opposite was the case with the expression of the superoxide anion.
YSG can significantly reduce MMS-induced OAZ in mice, which may be associated with oxidative stress.
探讨毓嗣颗粒（YSG）对甲磺酸甲酯（MMS）诱导的小鼠少弱精子症（OAZ）的治疗作用及作用机制。
将30只成年雄性小鼠随机分为数量相等的3组，即正常对照组、OAZ模型对照组和YSG干预组。通过口服MMS建立OAZ模型，YSG干预组的模型小鼠每天按0.144 g/100 g体重灌胃给予YSG混悬液，连续给药48天。然后，处死所有小鼠，摘取附睾检测精子数量和活力，通过苏木精-伊红（HE）染色观察生精小管形态，采用逆转录-聚合酶链反应（RT-PCR）检测生殖细胞、精子细胞、精母细胞、支持细胞及血睾屏障相关基因的表达，并检测血液中的氧化应激水平。
与正常对照组相比，OAZ模型小鼠的精子数量（［49.2±0.7］对［23.6±0.4］×10⁷/ml/g，P<0.05）和精子活力（［76.3±0.7］%对［5.0±5.8］%，P<0.05）显著降低，YSG干预后均显著升高（［38.4±0.5］×10⁷/ml/g和［71.5±0.5］%）（P<0.05）。OAZ模型小鼠还表现为生精小管退化萎缩、生精上皮变薄、不同层次细胞排列紊乱、管腔内精子数量减少以及附睾中生殖细胞层次不清，而YSG干预后的小鼠生精小管结构规则、排列有序且层次清晰。与正常对照组相比，OAZ模型组和YSG干预组中Vasa、Dazl和Snd1基因的表达显著降低（P<0.05），但Gfra、Plzf、Stra8、Spo11、Sycp3、Sox9和Vim基因的表达无显著变化（P>0.05）。与正常对照组相比，OAZ模型小鼠血清中的超氧化物歧化酶（SOD）活性显著降低（P<0.05），YSG干预后再次升高（P<0.05），但超氧阴离子的表达情况则相反。
YSG可显著减轻MMS诱导的小鼠OAZ，其机制可能与氧化应激有关。
