Yan Bin, Wang Fu, Gao Qing-He, Zhang Ji-Wei, Zhang Xiu-Ju, Yu Guo-Jin, Qiu Jun-Feng, Liu Yu, Liu Sheng-Jing, Guo Jun
Department of Andrology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
Graduate School, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhonghua Nan Ke Xue. 2019 Dec;25(12):1113-1117.
To explore the protective effect of the Chinese medicinal prescription Linggui Fang (LGF) on the reproductive system of the ornidazole-induced asthenospermia (AS) rat and its possible action mechanisms.
Forty male SD rats weighing 200－230 g were equally randomized into four groups, blank control, AS model control, LGF treatment and L-carnitine (LC) intervention. The AS models were made in the latter three groups by intragastrical administration of ornidazole at 400 mg/kg. Meanwhile, the rats in the LGF group were treated intragastrically with LGF at 17.5 g/kg, those in the LC group with LC at 100 mg/kg, and the control animals with 0.5% sodium carboxymethylcellulose (CMC-Na), all once a day for 4 successive weeks. Then, all the rats were sacrificed for examination of the semen parameters, determination of the LC content and OCTN2 mRNA expression in the epididymis and observation of the histopathological changes in the testis.
Compared with the AS model controls, the rats in the other groups showed significantly higher percentages of progressively motile sperm and total motile sperm (P < 0.01) as well as a higher LC content in the epididymis (P < 0.01), but no statistically significant difference in sperm concentration (P > 0.05). The expression of OCTN2 mRNA was remarkably upregulated in the LGF and LC groups in comparison with that in the AS model control (P < 0.05). Compared with the rats in the blank control group, the AS model controls exhibited markedly increased morphologically abnormal seminiferous tubules, irregularly arranged, with narrowed lumens and reduced numbers of sperm and sperm cells, as well as significantly increased hollow seminiferous tubules with deficient and disorderly arranged spermatogenic cells and partial epithelial degeneration and vacuolization. Those in the LGF and LC groups, however, manifested almost normal testicular histomorphology, with basically regular arrangement of different layers of seminiferous tubules.
①Ornidazole induces AS in rats by reducing the LC content in the epididymis, while LGF can improve the sperm motility and testicular morphology of the rats and upregulate the expression of OCTN2 mRNA in the epididymis by increasing the LC concentration.
探讨中药方剂灵桂方（LGF）对奥硝唑诱导的弱精子症（AS）大鼠生殖系统的保护作用及其可能的作用机制。
将40只体重200－230 g的雄性SD大鼠随机分为四组，即空白对照组、AS模型对照组、LGF治疗组和L-肉碱（LC）干预组。后三组通过灌胃给予400 mg/kg奥硝唑制备AS模型。同时，LGF组大鼠以17.5 g/kg的剂量灌胃给予LGF，LC组大鼠以100 mg/kg的剂量灌胃给予LC，对照组动物灌胃给予0.5%羧甲基纤维素钠（CMC-Na），均每日1次，连续4周。然后，处死所有大鼠，检测精液参数，测定附睾中LC含量和OCTN2 mRNA表达，并观察睾丸组织病理学变化。
与AS模型对照组相比，其他组大鼠的前向运动精子百分比和总活动精子百分比显著更高（P < 0.01），附睾中LC含量也更高（P < 0.01），但精子浓度无统计学显著差异（P > 0.05）。与AS模型对照组相比，LGF组和LC组中OCTN2 mRNA的表达明显上调（P < 0.05）。与空白对照组大鼠相比，AS模型对照组的生精小管形态异常明显增加，排列不规则，管腔狭窄，精子和精细胞数量减少，中空生精小管显著增加，生精细胞排列稀疏、紊乱，部分上皮变性和空泡化。然而，LGF组和LC组大鼠的睾丸组织形态学几乎正常，生精小管各层排列基本规则。
①奥硝唑通过降低附睾中LC含量诱导大鼠发生AS，而LGF可通过提高LC浓度改善大鼠精子活力和睾丸形态，并上调附睾中OCTN2 mRNA的表达。
