Masuoka N, Ubuka T, Kinuta M, Yoshida S, Taguchi T
Department of Biochemistry, Okayama University Medical School, Japan.
Acta Med Okayama. 1988 Oct;42(5):247-52. doi: 10.18926/AMO/31022.
A new gas chromatographic method for the determination of sulfate was developed. In this method, sulfate was quantitatively converted to a volatile derivative, dimethyl sulfate, by a two-step procedure. First, sulfate was converted to silver sulfate by reaction with silver oxide, and then to dimethyl sulfate by reaction with methyl iodide. The derivative was analyzed by gas chromatography. Methyl methanesulfonate was used as an internal standard. The method was applied to the determination of total urinary sulfate. Phosphate and chloride ions, which interfered with the present method, were eliminated with the use of basic magnesium carbonate and an excess of silver oxide, respectively. Recovery was over 96% when 5 to 40 mumol/ml of sulfate was added to human urine samples.
开发了一种测定硫酸盐的新气相色谱法。在该方法中，通过两步程序将硫酸盐定量转化为挥发性衍生物硫酸二甲酯。首先，硫酸盐通过与氧化银反应转化为硫酸银，然后通过与甲基碘反应转化为硫酸二甲酯。通过气相色谱分析该衍生物。甲磺酸甲酯用作内标。该方法用于测定尿中总硫酸盐。分别使用碱式碳酸镁和过量的氧化银消除干扰本方法的磷酸根离子和氯离子。向人尿样中添加5至40μmol/ml的硫酸盐时，回收率超过96%。
