Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis / College of Veterinary Medicine, Jilin University, Changchun, China.
Panjin Inspection and Testing Center, Panjin, China.
J Interferon Cytokine Res. 2020 May;40(5):268-278. doi: 10.1089/jir.2019.0182. Epub 2020 Apr 1.
Interleukin-1 receptor antagonist (IL-1Ra) is an antagonist of IL-1β binding IL-1β receptors but does not induce intracellular responses or signal transduction. In this study, the full-length complementary DNA (cDNA) of the gene () was identified from sheep () using rapid amplification of cDNA ends PCR and submitted to GenBank with the accession number KC425613. The cDNA comprised an open reading frame of 525 bp encoding a protein of 19765.8 Da, a 5'-untranslated region (UTR) of 27 bp, and a 3'-UTR of 676 bp with a poly(A) tail. Recombinant OaIL-1Ra with bioactivity was expressed in a prokaryotic expression system, and a monoclonal antibody against native OaIL-1Ra was prepared. Through Western blot analyses, the OaIL-1Ra protein was widely expressed in lung, heart, spleen, liver, kidney, muscle, intestine, lymphonodi, rumen, and white blood cells, with the highest levels in liver and spleen. The expression of OaIL-1Ra in primary cultured white blood cells of sheep were highly induced in a time-dependent manner when challenged with different bacteria. These results implied that is associated with immune responses during bacterial infections.
白细胞介素 1 受体拮抗剂（IL-1Ra）是白细胞介素 1β（IL-1β）结合白细胞介素 1 受体的拮抗剂，但不会诱导细胞内反应或信号转导。本研究采用快速扩增 cDNA 末端 PCR 技术，从绵羊中鉴定出全长 cDNA（），并将其提交至 GenBank，登录号为 KC425613。该 cDNA 包含一个 525bp 的开放阅读框，编码一个 19765.8Da 的蛋白质，一个 27bp 的 5'-非翻译区（UTR）和一个 676bp 的 3'-UTR，带有 poly(A)尾巴。具有生物活性的重组 OaIL-1Ra 在原核表达系统中表达，并制备了针对天然 OaIL-1Ra 的单克隆抗体。通过 Western blot 分析，OaIL-1Ra 蛋白在肺、心、脾、肝、肾、肌肉、肠、淋巴结、瘤胃和白细胞中广泛表达，在肝和脾中表达水平最高。绵羊原代培养白细胞在受到不同细菌刺激时，OaIL-1Ra 的表达呈时间依赖性高度诱导。这些结果表明，与细菌感染期间的免疫反应有关。
