Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, China-Japan Union Hospital, The First Hospital, Jilin University, Xi An Da Lu 5333, Changchun 130062, China.
Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, China-Japan Union Hospital, The First Hospital, Jilin University, Xi An Da Lu 5333, Changchun 130062, China; Panjin Inspection and Testing Center, Panjin 124010, China.
Microb Pathog. 2018 Mar;116:180-188. doi: 10.1016/j.micpath.2018.01.011. Epub 2018 Jan 10.
The interleukin-1 family is an important component of the innate immune system and plays an important role in regulating immune responses on the invasion of intracellular parasites in the acquired immune system. Interleukin 1β (IL-1β) is one of the members of the IL-1 family that predominantly activates downstream signaling pathways to play immunological functions of stimulating T and B lymphocyte activation and promoting the various syntheses of inflammatory substances in conjunction with other cytokines. Here, a full-length IL-1β cDNA (OaIL-1β) of sheep (Ovis aries) was cloned using rapid amplification of cDNA ends (RACE), which consists of 1494 bp and contains a 5'-UTR region with a length of 83 bp, a complete ORF of 801 bp in length, and a 3'-UTR region with a length of 642 bp. Recombinant protein OaIL-1β was expressed and purified, and the monoclonal antibody against IL-1β of sheep is prepared. Western blotting results showed that the sheep IL-1β protein was detected in the heart, liver, lung, kidney, stomach, intestine, muscle, lymph nodes and leukocytes with the highest expression in the muscle and the lowest expression in the lung. Different bacteria treating sheep white blood cells induced differential expression of OaIL-1β. Compared with the normal sheep, OaIL-1β in the buffy coat was differentially expressed in the Brucella melitensis-challenged group and the B. suis S2 strain-inoculated group. However, whether IL-1β may be considered as a molecular biomarker for differing Brucella-infected animals from brucellosis-vaccinated animals or not need to be further studied.
白细胞介素-1 家族是先天免疫系统的重要组成部分，在调节获得性免疫系统中细胞内寄生虫入侵时的免疫反应方面发挥着重要作用。白细胞介素 1β（IL-1β）是白细胞介素 1 家族的成员之一，主要通过激活下游信号通路发挥免疫功能，刺激 T 和 B 淋巴细胞的激活，并与其他细胞因子一起促进各种炎症物质的合成。在这里，使用快速扩增 cDNA 末端（RACE）克隆了绵羊（Ovis aries）的全长 IL-1β cDNA（OaIL-1β），该 cDNA 由 1494bp 组成，包含一个 5'-UTR 区，长度为 83bp，一个完整的 ORF 长 801bp，和一个 3'-UTR 区，长度为 642bp。表达和纯化了重组蛋白 OaIL-1β，并制备了针对绵羊 IL-1β的单克隆抗体。Western blot 结果表明，绵羊 IL-1β蛋白在心脏、肝脏、肺、肾脏、胃、肠、肌肉、淋巴结和白细胞中被检测到，在肌肉中的表达最高，在肺中的表达最低。不同的细菌处理绵羊白细胞诱导了 OaIL-1β的差异表达。与正常绵羊相比，在布氏菌属 melitensis 攻毒组和 B. suis S2 株接种组的白细胞层中，OaIL-1β 差异表达。然而，IL-1β是否可以被认为是区分布鲁氏菌感染动物和布鲁氏菌病疫苗接种动物的分子生物标志物，还需要进一步研究。
