Department of Geriatric Respiratory and Sleep, Henan Institute of Respiratory Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.
Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.
Mol Cell Probes. 2020 Aug;52:101566. doi: 10.1016/j.mcp.2020.101566. Epub 2020 Mar 28.
This study aims to evaluate the role of the EPHA5 mutation in the migration and invasion of non-small cell lung cancer (NSCLC) cells and in modulating the killing effect of natural killer (NK) cells to NSCLC cells.
EPHA5-wt (wild type) and EPHA5-mut (mutation) plasmids were constructed. EPHA5 was silenced using si-EPHA5. NSCLC cell migration and invasion were determined using Transwell assays. NK cell proliferation and apoptosis were determined using CCK-8 assay and flow cytometry, respectively. The killing effect of NK cells to NSCLC cells was also examined.
EPHA5 mutation significantly promoted migration and invasion in NSCLC cells. Furthermore, EPHA5 mutation notably impaired the cytotoxicity of NK cells against NSCLC cells. In contrast, EPHA5-wt overexpression and EPHA5 silencing exerted the opposite effect.
EPHA5 mutation impairs the NK cell-mediated cytotoxicity against NSCLC cells and promotes migration and invasion in NSCLC cells.
本研究旨在评估 EphA5 突变在非小细胞肺癌（NSCLC）细胞迁移和侵袭中的作用，以及调节自然杀伤（NK）细胞对 NSCLC 细胞杀伤作用的机制。
构建 EphA5-wt（野生型）和 EphA5-mut（突变型）质粒。使用 si-EPHA5 沉默 EphA5。通过 Transwell 实验检测 NSCLC 细胞的迁移和侵袭。使用 CCK-8 法和流式细胞术分别检测 NK 细胞的增殖和凋亡。还检测了 NK 细胞对 NSCLC 细胞的杀伤作用。
EphA5 突变显著促进了 NSCLC 细胞的迁移和侵袭。此外，EphA5 突变明显削弱了 NK 细胞对 NSCLC 细胞的细胞毒性。相比之下，EphA5-wt 过表达和 EphA5 沉默则产生了相反的效果。
EphA5 突变削弱了 NK 细胞对 NSCLC 细胞的杀伤作用，并促进了 NSCLC 细胞的迁移和侵袭。
