Department of Neonatology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China.
Department of Pediatric, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China.
Mol Cell Probes. 2020 Aug;52:101565. doi: 10.1016/j.mcp.2020.101565. Epub 2020 Mar 29.
This study aimed to test the hypothesis that long non-coding RNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) could exacerbate brain injury caused by intrauterine infection in neonatal rats.
Intrauterine infection was induced in pregnant rats by lipopolysaccharide (LPS). After delivery, newborn rats with brain injury caused by intrauterine infection were randomly divided into control, control shRNA, and CRNDE shRNA groups. CRNDE expression in serum and amniotic fluid of pregnant rats and neonatal brain tissues were determined by quantitative real-time PCR (qRT-PCR). Morris water maze (MWM) task was used to test the spatial learning and memory ability. Histological examination and apoptosis detection were performed by hematoxylin and eosin (H&E) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Immunohistochemistry was conducted to evaluate the activation of astrocytes and microglia.
LncRNA CRNDE was highly expressed in serum and amniotic fluid of maternal rats and in brain tissues of offspring rats. Furthermore, shRNA-mediated CRNDE downregulation could rescue the spatial learning and memory ability, improve brain histopathological changes and cell death, and inhibit the activation of astrocytes and microglia caused by LPS.
CRNDE silencing possessed a cerebral protective effect in neonatal rats with brain injury caused by interauterine infection.
本研究旨在验证长链非编码 RNA（lncRNA）结直肠肿瘤差异表达（CRNDE）是否会加剧宫内感染新生大鼠的脑损伤。
通过脂多糖（LPS）诱导妊娠大鼠宫内感染。宫内感染后，将新生大鼠随机分为对照组、对照 shRNA 组和 CRNDE shRNA 组。通过定量实时 PCR（qRT-PCR）检测母鼠血清和羊水以及新生鼠脑组织中 CRNDE 的表达。通过 Morris 水迷宫（MWM）任务测试空间学习和记忆能力。通过苏木精和伊红（H&E）和末端脱氧核苷酸转移酶 dUTP 缺口末端标记（TUNEL）染色分别进行组织学检查和细胞凋亡检测。免疫组织化学法评估星形胶质细胞和小胶质细胞的激活。
lncRNA CRNDE 在母鼠血清和羊水以及子鼠脑组织中高表达。此外，shRNA 介导的 CRNDE 下调可挽救 LPS 引起的空间学习和记忆能力下降，改善脑组织病理学变化和细胞死亡，并抑制星形胶质细胞和小胶质细胞的激活。
CRNDE 沉默对宫内感染新生大鼠脑损伤具有脑保护作用。
