Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing 100193, PR China; College of Food Science and Nutritional Engineering, National Engineering Research Centre for Fruits and Vegetables Processing, Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, China Agricultural University, Beijing 100083, PR China.
College of Food Science and Nutritional Engineering, National Engineering Research Centre for Fruits and Vegetables Processing, Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, China Agricultural University, Beijing 100083, PR China.
Food Chem. 2020 Aug 1;320:126648. doi: 10.1016/j.foodchem.2020.126648. Epub 2020 Mar 18.
High circulating branched-chain amino acid (BCAA) levels can be diagnosis indicators for obesity. Luffa cylindrica (luffa) is one of vegetables against obesity. However, whether the anti-obesity of luffa is associated with BCAA metabolism and gut microbiota remains unknown. Here, we used conventionally raised diet-induced obese (DIO) mice to prove dietary luffa could reduce higher circulating BCAA levels and upregulate the tissue-specific expressions of BCAA-catabolizing enzymes. Meanwhile, dietary luffa selectively decreased the relative abundances of g_Enterortabdus, g_Eubacterium_xylanophilum_group and g_Butyricicoccus that exhibited significantly positive correlations with BCAA levels, BMI and HOMA-IR. Bacterial functionality prediction indicated dietary luffa potentially inhibited bacterial BCAA biosynthesis for reducing BCAAs supplementation. More importantly, dietary luffa had no impacts on BCAA catabolism in germ-free-mimic DIO mice. Thus, dietary luffa improved BCAA dysfunction via gut microbiota to attenuate obesity. This study offers a novel insight into dietary intervention against obesity from the aspect of gut microbiota-amino acid metabolism.
高循环支链氨基酸（BCAA）水平可作为肥胖的诊断指标。丝瓜（丝瓜）是一种抗肥胖的蔬菜。然而，丝瓜的抗肥胖作用是否与 BCAA 代谢和肠道微生物群有关尚不清楚。在这里，我们使用常规饲养的饮食诱导肥胖（DIO）小鼠来证明饮食中的丝瓜可以降低较高的循环 BCAA 水平，并上调 BCAA 分解代谢酶的组织特异性表达。同时，饮食中的丝瓜选择性地降低了 g_Enterortabdus、g_Eubacterium_xylanophilum_group 和 g_Butyricicoccus 的相对丰度，这些菌与 BCAA 水平、BMI 和 HOMA-IR 呈显著正相关。细菌功能预测表明，饮食中的丝瓜可能通过抑制细菌 BCAA 生物合成来减少 BCAAs 的补充。更重要的是，饮食中的丝瓜对无菌模拟 DIO 小鼠的 BCAA 分解代谢没有影响。因此，饮食中的丝瓜通过肠道微生物群改善了 BCAA 功能障碍，从而减轻了肥胖。这项研究从肠道微生物群-氨基酸代谢的角度为饮食干预肥胖提供了新的见解。
