Department of Plant Physiology, Rheinisch-Westfälische Technische Hochschule Aachen (RWTH Aachen University), Aachen, Germany
Department of Botany, Rheinisch-Westfälische Technische Hochschule Aachen (RWTH Aachen University), Aachen, Germany.
Life Sci Alliance. 2020 Mar 31;3(5). doi: 10.26508/lsa.202000670. Print 2020 May.
The common foodstuff garlic produces the potent antibiotic defense substance allicin after tissue damage. Allicin is a redox toxin that oxidizes glutathione and cellular proteins and makes garlic a highly hostile environment for non-adapted microbes. Genomic clones from a highly allicin-resistant (AR-1), which was isolated from garlic, conferred allicin resistance to and even to Resistance-conferring genes had redox-related functions and were on core fragments from three similar genomic islands identified by sequencing and in silico analysis. Transposon mutagenesis and overexpression analyses revealed the contribution of individual candidate genes to allicin resistance. Taken together, our data define a multicomponent resistance mechanism against allicin in AR-1, achieved through horizontal gene transfer.
大蒜在组织损伤后会产生强效抗生素防御物质大蒜素。大蒜素是一种氧化还原毒素，可氧化谷胱甘肽和细胞蛋白，使大蒜对不适应的微生物具有高度敌意的环境。从大蒜中分离出的高度耐大蒜素的（AR-1）的基因组克隆赋予了对 和 的大蒜素抗性。抗性赋予基因具有氧化还原相关功能，并且位于通过测序和计算机分析鉴定的三个类似基因组岛的核心片段上。转座子诱变和过表达分析揭示了单个候选基因对大蒜素抗性的贡献。总之，我们的数据定义了 AR-1 中针对大蒜素的多组分抗性机制，这是通过水平基因转移实现的。
