Azab Sandi M, de Souza Russell J, Teo Koon K, Anand Sonia S, Williams Natalie C, Holzschuher Jordan, McGlory Chris, Philips Stuart M, Britz-McKibbin Philip
Departments of Chemistry and Chemical Biology, McMaster University, Hamilton, ON, Canada; Department of Pharmacognosy, Alexandria University, Alexandria, Egypt.
Medicine, McMaster University, Hamilton, ON, Canada; Health Research Methods, Evidence, and Impact, McMaster University, Hamilton, ON, Canada.
J Lipid Res. 2020 Jun;61(6):933-944. doi: 10.1194/jlr.D120000630. Epub 2020 Mar 31.
Nutritional studies rely on various biological specimens for FA determination, yet it is unclear how levels of serum NEFAs correlate with other circulating lipid pools. Here, we used a high-throughput method (<4 min/sample) based on multisegment injection-nonaqueous capillary electrophoresis-mass spectrometry (MSI-NACE-MS) to investigate whether specific serum NEFAs have utility as biomarkers of dietary fat intake in women. We first identified circulating NEFAs correlated with long-term/habitual food intake among pregnant women with contrasting dietary patterns ( = 50). Acute changes in serum NEFA trajectories were also studied in nonpregnant women ( = 18) following high-dose (5 g/day) fish oil (FO) supplementation or isoenergetic sunflower oil placebo over 56 days. In the cross-sectional study, serum ω-3 FAs correlated with self-reported total ω-3 daily intake, notably EPA as its NEFA ( = 0.46; = 0.001), whereas pentadecanoic acid was associated with full-fat dairy intake ( = 0.43; = 0.002), outcomes consistent with results from total FA serum hydrolysates. In the intervention cohort, serum ω-3 NEFAs increased 2.5-fold from baseline within 28 days following FO supplementation, and this increase was most pronounced for EPA ( = 0.0004). Unlike for DHA, circulating EPA as its NEFA also strongly correlated to EPA concentrations measured from erythrocyte phospholipid hydrolysates ( = 0.66; = 4.6 × 10) and was better suited to detect dietary nonadherence. We conclude that MSI-NACE-MS offers a rapid method to quantify serum NEFAs and objectively monitor dietary fat intake in women that is complementary to food-frequency questionnaires.
营养研究依靠各种生物样本进行脂肪酸（FA）测定，但血清非酯化脂肪酸（NEFAs）水平与其他循环脂质池之间的关联尚不清楚。在此，我们使用了一种基于多段进样-非水毛细管电泳-质谱联用（MSI-NACE-MS）的高通量方法（<4分钟/样本），来研究特定血清NEFAs是否可作为女性膳食脂肪摄入的生物标志物。我们首先在饮食模式不同的孕妇（n = 50）中，确定了与长期/习惯性食物摄入相关的循环NEFAs。还对18名非孕妇在高剂量（5克/天）鱼油（FO）补充或等能量葵花籽油安慰剂干预56天后血清NEFA轨迹的急性变化进行了研究。在横断面研究中，血清ω-3脂肪酸与自我报告的每日总ω-3摄入量相关，特别是二十碳五烯酸（EPA）作为其NEFA（r = 0.46；P = 0.001），而十五烷酸与全脂乳制品摄入量相关（r = 0.43；P = 0.002），这些结果与总脂肪酸血清水解产物的结果一致。在干预队列中，补充FO后28天内，血清ω-3 NEFAs从基线水平增加了2.5倍，其中EPA的增加最为明显（P = 0.0004）。与二十二碳六烯酸（DHA）不同，作为NEFA的循环EPA也与红细胞磷脂水解产物中测得的EPA浓度密切相关（r = 0.66；P = 4.6×10⁻⁶），并且更适合检测饮食不依从情况。我们得出结论，MSI-NACE-MS提供了一种快速定量血清NEFAs的方法，可客观监测女性的膳食脂肪摄入，是对食物频率问卷的补充。
