Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, Germany.
Department of Otorhinolaryngology, University Hospital, LMU Munich, Munich, Germany.
Anticancer Res. 2020 Apr;40(4):2117-2123. doi: 10.21873/anticanres.14170.
BACKGROUND/AIM: The incidence of human papilloma virus (HPV)-related head and neck squamous cell carcinoma (HNSCC) has been increasing in the last decades. Analysis of oral brushing or rinsing samples for screening or stratification could potentially improve screening and prevention.
Oral brushes and mouthwashes were taken from 20 patients with HPV-associated HNSCC before definite therapy. HPV genotyping was performed for the detection of 14 high-risk HPV subtypes and correlated to DNA isolated from tumor tissue.
Ten of 20 patients were tested HPV positive by using either method. There was a significant correlation between macroscopic visibility of tumor and positive HPV detection (p<0.001) and HPV detection and tumor size (p<0.001). HPV was detected in all macroscopically visible tumors. Half of the HPV cases who had macroscopically invisible tumors were missed by both methods.
Both techniques are limited in the detection of macroscopically non-visible and small tumors. Therefore, the application of these techniques for screening or diagnosis of HNSCC is not recommended.
背景/目的：在过去几十年中，人乳头瘤病毒（HPV）相关的头颈部鳞状细胞癌（HNSCC）的发病率一直在上升。对口腔刷或漱口样本进行分析以进行筛查或分层，可能有助于改善筛查和预防。
在明确治疗前，从 20 例 HPV 相关 HNSCC 患者中采集了口腔刷和漱口水。进行 HPV 基因分型以检测 14 种高危 HPV 亚型，并与肿瘤组织中分离的 DNA 进行相关性分析。
20 例患者中有 10 例通过两种方法检测出 HPV 阳性。肿瘤的宏观可见性与 HPV 检测的阳性（p<0.001）和 HPV 检测与肿瘤大小（p<0.001）之间存在显著相关性。所有宏观可见的肿瘤均检测到 HPV。在宏观上看不见肿瘤的 HPV 病例中，有一半被两种方法都漏诊。
两种技术在检测宏观上不可见和小肿瘤方面均存在局限性。因此，不建议将这些技术应用于 HNSCC 的筛查或诊断。
