Centre for Biotechnology and Microbiology, University of Swat, Mingora 19200, Pakistan.
Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Peshawar 25120, Pakistan.
Molecules. 2021 Dec 21;27(1):14. doi: 10.3390/molecules27010014.
is an important medicinal plant that belongs to the family. The leaves of are a rich source of many health-promoting agents such as polyphenols, flavonoids, and steviol glycoside, which play a key role in controlling obesity and diabetes. New strategies such as the elicitation of culture media are needed to enhance the productivity of active components. Herein, the extracts were exploited as elicitors to enhance the productivity of active components. is one of the parasitic plants that has the ability to elongate very fast and cover the host plant. Consequently, it may be possible that the addition of extracts to adventitious root cultures (ADR) of may elongate the root more than control cultures to produce higher quantities of the desired secondary metabolites. Therefore, the main objective of the current study was to investigate the effect of extract as a biotic elicitor on the biomass accumulation and production of antioxidant secondary metabolite in submerged adventitious root cultures of . Ten different concentrations of were added to liquid media containing 0.5 mg/L naphthalene acetic acid (NAA). The growth kinetics of adventitious roots was investigated for a period of 49 days with an interval of 7 days. The maximum biomass accumulation (7.83 g/3 flasks) was observed on medium containing 10 mg/L extract of on day 49. As the concentration of extract increases in the culture media, the biomass gradually decreases after 49 days of inoculation. In this study, the higher total phenolics content (0.31 mg GAE/g-DW), total flavonoids content (0.22 mg QE/g-DW), and antioxidant activity (85.54%) were observed in 100 mg/L treated cultures. The higher concentration (100 mg/L) of extract considerably increased the total phenolics content (TPC), total phenolics production (TPP), total flavonoids content (TFC), total flavonoids production (TFP), total polyphenolics content (TPPC), and total polyphenolics production (TPPP). It was concluded that the extract of moderately improved biomass accumulation but enhanced the synthesis of phenolics, flavonoids, and antioxidant activities. Here, biomass's independent production of secondary metabolites was observed with the addition of extract. The present study will be helpful to scale up adventitious roots culture into a bioreactor for the production of secondary metabolites rather than biomass accumulation in medicinally important .
是一种重要的药用植物，属于 科。 的叶子是许多促进健康的物质的丰富来源，如多酚、类黄酮和甜菊糖苷，它们在控制肥胖和糖尿病方面起着关键作用。需要新的策略，如培养介质的激发，以提高活性成分的产量。本文利用 提取物作为激发子来提高活性成分的产量。 是一种寄生植物，具有快速伸长的能力，并覆盖宿主植物。因此，向 不定根培养物（ADR）中添加 提取物可能会使根比对照培养物伸长更多，从而产生更多所需的次生代谢物。因此，本研究的主要目的是研究 提取物作为生物激发子对 浸提不定根培养物生物量积累和抗氧化次生代谢产物生产的影响。在含有 0.5mg/L 萘乙酸（NAA）的液体培养基中添加了十种不同浓度的 提取物。在 49 天的时间内，每隔 7 天就会对不定根的生长动力学进行研究。在第 49 天，在含有 10mg/L 提取物的培养基中观察到最大的生物量积累（7.83g/3 瓶）。随着培养介质中提取物浓度的增加，接种后 49 天，生物量逐渐减少。在这项研究中，在 100mg/L 处理培养物中观察到总酚含量（0.31mgGAE/g-DW）、总类黄酮含量（0.22mgQE/g-DW）和抗氧化活性（85.54%）较高。较高浓度（100mg/L）的 提取物显著增加了总酚含量（TPC）、总酚产量（TPP）、总类黄酮含量（TFC）、总类黄酮产量（TFP）、总多酚含量（TPPC）和总多酚产量（TPPP）。研究结论认为， 提取物适度地提高了生物量的积累，但增强了酚类、类黄酮和抗氧化活性的合成。在这里，观察到添加提取物后生物量独立地产生次生代谢物。本研究将有助于将不定根培养物扩大到生物反应器中，以生产次生代谢物，而不是在药用 中积累生物量。
