Moussa Ashaimaa Y, Lambert Christopher, Stradal Theresia E B, Ashrafi Samad, Maier Wolfgang, Stadler Marc, Helaly Soleiman E
Department of Microbial Drugs, Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.
Department of Pharmacognosy, Faculty of Pharmacy, Ain shams University, Abbassia, 11566 Cairo, Egypt.
Antibiotics (Basel). 2020 Mar 22;9(3):132. doi: 10.3390/antibiotics9030132.
Fungal associations with nematodes have attracted scientific attention because of the need to develop new biocontrol agents. In this context, , an antagonistic fungus previously isolated from the plant parasitic cyst nematode , was selected to carry out an in-depth metabolomic study for its active metabolites. Herein, three new nonapeptide peptaibols with leucinostatin based sequences were isolated and identified by 1, 2D NMR, and HR-ESI-MS-MS. The absolute configuration was assigned based on Marfay's analysis and Mosher ester formation. The new leucinostatins manifested moderate nematicidal effect against the plant pathogenic nematode with LD values ranging from 5 to 7 µg/mL. Furthermore, a cyclodepsipeptide, named arthrichitin D, with five amino acid residues attached to a 3-hydroxy-2,4-dimethylhexadeca-4,6-dienoic fatty acid chain was discovered and showed weak nematicidal effect against . Chaetoglobosin B and its 19--acetyl derivative were also obtained as minor metabolites, and the activity of chaetoglobosin B on the actin cytoskeleton of mammalian cells was assessed.
由于需要开发新的生物防治剂，真菌与线虫的关联已引起科学关注。在此背景下，一种先前从植物寄生性胞囊线虫中分离出的拮抗真菌，被选来对其活性代谢产物进行深入的代谢组学研究。在此，通过一维、二维核磁共振以及高分辨电喷雾电离质谱-质谱，分离并鉴定出了三种基于亮抑菌素序列的新型九肽类脂肽。基于马尔费分析和莫舍尔酯的形成确定了其绝对构型。新型亮抑菌素对植物病原线虫表现出中等的杀线虫活性，其半数致死剂量值在5至7微克/毫升之间。此外，还发现了一种环缩肽，命名为节曲菌素D，它有五个氨基酸残基连接在一条3-羟基-2,4-二甲基十六碳-4,6-二烯酸脂肪酸链上，并且对该线虫表现出较弱的杀线虫活性。还获得了球毛壳菌素B及其19-乙酰衍生物作为次要代谢产物，并评估了球毛壳菌素B对哺乳动物细胞肌动蛋白细胞骨架的活性。
