College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, P. R. China.
Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, P. R. China.
FEMS Microbiol Lett. 2019 Jul 1;366(14). doi: 10.1093/femsle/fnz169.
Management of nematodes is a very hectic job due to a highly diverse group of organisms. To find lead compounds for new nematicide development, five metabolites (1-5) were isolated from the culture broth of Chaetomium globosum YSC5 and tested for nematicidal activities against the second stage juveniles (J2s) of Meloidogyne javanica. The results revealed that chaetoglobosin A (1), chaetoglobosin B (2) and flavipin (3) exhibited strong adverse effects (91.6, 83.8 and 87.4%, respectively) on J2 mortality at 200 μg/mL with LC50 values of 88.4, 107.7 and 99.2 μg/mL after 72 h, respectively, while 3-methoxyepicoccone (4) and 4,5,6-trihydroxy-7-methylphthalide (5) showed moderate effects (78.0 and 75.5%, respectively) with LC50 values of 124.0 and 131.6 μg/mL, respectively. Furthermore, in pot assay compounds 1 and 2 appeared to be promising metabolites at 200 μg/mL that significantly reduced nematode reproduction and showed a positive influence on plant growth. Our findings could be helpful for development of new potential bio-based pesticides for integrated management of plant-parasitic nematode.
由于线虫的生物多样性很高，因此对线虫的管理是一项非常繁忙的工作。为了寻找新杀线虫剂开发的先导化合物，从 Chaetomium globosum YSC5 的发酵液中分离得到了 5 种代谢产物（1-5），并测试了它们对线虫第二阶段幼虫（J2）的杀线虫活性。结果表明，在 200μg/mL 时，Chaetoglobosin A（1）、Chaetoglobosin B（2）和 Flavipin（3）对线虫死亡率有很强的不良影响（分别为 91.6%、83.8%和 87.4%），LC50 值分别为 88.4、107.7 和 99.2μg/mL，72 h 后；而 3-甲氧基表甾酮（4）和 4,5,6-三羟基-7-甲基邻苯二甲酸内酯（5）表现出中等活性（分别为 78.0%和 75.5%），LC50 值分别为 124.0 和 131.6μg/mL。此外，在盆栽试验中，化合物 1 和 2 在 200μg/mL 时表现出良好的杀线虫活性，显著降低了线虫的繁殖，并对植物生长表现出积极的影响。我们的研究结果可能有助于开发新的潜在基于生物的农药，以实现植物寄生线虫的综合管理。
