Haagsma H, ter Laak E A
Tijdschr Diergeneeskd. 1977 Apr 1;102(7):429-36.
The second outbreak of botulism in broiler chickens diagnosed in the Netherlands is reported. In this case, the source of botulinum toxin was positively located. Despite the fact that the owner had emphatically assured that all sick and dead broilers had been carefully removed, several carcasses of broilers, almost unrecognizable at first sight, were found to be present in the litter covering the floor, particularly at the rear of the fowl house. The concentration of toxin in the carcasses in the area surrounding this source of infection averaged well over 1,000,000 LD50 per gram of tissue. This is more than sufficient to account for the outbreak of botulism as the oral MLD of the strain of Cl. botulinum type C isolated in previous studied had been found to be well over 4,000,000 LD50 per kg of body weight for chickens, the oral MLD of the strain of Cl. botulinum type C isolated in the second outbreak being found to be 2,000,000 LD50 per kg. of body weight. In discussing the origin of the infection, attention is finally drawn to the possible relationship with the fact that botulism has become increasingly common among waterfowl in the Netherlands since 1970.
据报道，荷兰确诊了肉鸡肉毒中毒的第二次爆发。在这种情况下，肉毒杆菌毒素的来源已被明确确定。尽管鸡场主再三保证已将所有病鸡和死鸡仔细清理，但仍发现地面垫料中有几具肉鸡尸体，乍一看几乎辨认不出，尤其是在禽舍后部。感染源周围区域的尸体中毒素浓度平均每克组织超过1000000 LD50。这足以解释肉毒中毒的爆发，因为在之前的研究中发现，分离出的C型肉毒杆菌菌株对鸡的口服半数致死剂量（MLD）远超过每千克体重4000000 LD50，而在第二次爆发中分离出的C型肉毒杆菌菌株的口服MLD为每千克体重2000000 LD50。在讨论感染源时，最后提到了自1970年以来肉毒中毒在荷兰水禽中越来越普遍这一事实可能存在的关联。
