Powers B E, Norrdin R W, Snyder S P, Smith R E
Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
Am J Vet Res. 1988 Sep;49(9):1589-97.
Ten-day-old chicken embryos were inoculated with isolates of myeloblastosis-associated virus that induced osteopetrosis of slow or rapid onset. Bursa of Fabricius, thymus, spleen, bone marrow, kidney, liver, and lung were examined at 15, 17, and 19 days in ovo and at 7 and 25 days after hatching by histologic and immunoperoxidase techniques. Tissues from 19-day-old in ovo embryos also were examined by electron microscopy. The lymphoid organs of embryos inoculated with all isolates manifested changes suggesting inhibited development. Virus was most often associated with macrophages, heterophils, and nonlymphoid stromal cells in these organs. Viral particles and antigen were abundant in tissues from embryos inoculated with slow-onset isolates, but cell necrosis was infrequent. The kidney and bursa had especially abundant viral particles and antigen. Conversely, viral particles and antigen were minimal in tissues from embryos inoculated with the rapid-onset isolate, yet intravascular cellular thrombi, substantial cell necrosis, and increased heterophils and hemocytoblasts were found.
将10日龄鸡胚接种与引发缓慢或快速发作的骨髓性白血病相关病毒的分离株。在孵化15、17和19天以及孵化后7和25天，通过组织学和免疫过氧化物酶技术检查法氏囊、胸腺、脾脏、骨髓、肾脏、肝脏和肺。对19日龄孵化中胚胎的组织也进行电子显微镜检查。接种所有分离株的胚胎的淋巴器官均表现出提示发育受抑制的变化。病毒最常与这些器官中的巨噬细胞、嗜异性粒细胞和非淋巴间质细胞相关。接种缓慢发作分离株的胚胎组织中病毒颗粒和抗原丰富，但细胞坏死罕见。肾脏和法氏囊中病毒颗粒和抗原尤其丰富。相反，接种快速发作分离株的胚胎组织中病毒颗粒和抗原极少，但发现血管内细胞血栓、大量细胞坏死以及嗜异性粒细胞和成血细胞增加。
