急性胃肠炎患儿中腺病毒40型和41型的分子研究

Molecular Study of Adenovirus Genotypes 40 and 41 in Children with Acute Gastroenteritis.

作者信息

Essa Sara G, Zaki Maysaa El Sayed, Elmansoury Eman A, Hassan Rasha H, El Kheir Nermin Y A

机构信息

Department of Clinical Pathology, Mansoura Faculty of Medicine, Mansoura University, Mansoura, Egypt.

Department of Medical Microbiology and Immunology, Mansoura Faculty of Medicine, Mansoura University, Mansoura, Egypt.

出版信息

Infect Disord Drug Targets. 2022;22(8):79-83. doi: 10.2174/1871526522666220509054535.

DOI:10.2174/1871526522666220509054535

PMID:35532250

Abstract

BACKGROUND

Adenovirus is a common virus associated with acute gastroenteritis in children. There are certain genotypes that are prevalent in these infections, such as genotypes 40 and 41.

OBJECTIVE

The aim of the present study was to investigate the incidence of adenovirus genotypes 40 and 41 in children with acute gastroenteritis by polymerase chain reaction (PCR) and also to determine the possibility of Adenovirus co-infections with Rotavirus.

METHODS

The study was a cross-sectional study that included 100 children with acute gastroenteritis. The children were subjected to full history taking and clinical examination. Stool samples from the patients were subjected to detection of adenovirus and rotavirus antigens by enzyme-linked immunosorbent assay (ELISA) and detection of adenovirus genotypes 40 and 41 by polymerase chain reaction (PCR).

RESULTS

The most prevalent virus by the used methods was rotavirus antigen in the stool (35%). Adenovirus antigen detection was positive in 23% of the stool samples, with positive PCR for these samples in 22%. The ADv40 was detected in 13 samples, and ADv41 was detected in 9 samples. One positive sample by adenovirus antigen ELISA was negative by PCR for these genotypes. Mixed rotavirus and adenovirus by ELISA were detected in 7% of the children. In patients with positive adenovirus antigen by ELISA, the most common symptoms were vomiting (54.5%) and abdominal pain (45.5%). An insignificant difference between fever (P=0.94) and abdominal pain (P=0.63) was detected in children infected with adenovirus compared to patients infected with other organisms. The adenovirus was detected in 68.2% of children with acute gastroenteritis ≤ 24 months. Vomiting was significantly increased in children with adenovirus (54.5%) compared to children negative for adenovirus (23.1%-P=0.004, OR 4.0, 95%CI: 1.5-10.8).

CONCLUSION

The study highlights the presence of adenovirus genotypes 40 and 41 in the stool of children with acute gastroenteritis. Combined rotavirus and adenovirus infections were detected in our study.

摘要

背景

腺病毒是一种与儿童急性胃肠炎相关的常见病毒。某些基因型在这些感染中较为普遍，如40型和41型。

目的

本研究旨在通过聚合酶链反应（PCR）调查急性胃肠炎患儿中腺病毒40型和41型的发生率，并确定腺病毒与轮状病毒合并感染的可能性。

方法

本研究为横断面研究，纳入100例急性胃肠炎患儿。对患儿进行全面的病史采集和临床检查。采用酶联免疫吸附测定（ELISA）检测患者粪便样本中的腺病毒和轮状病毒抗原，采用聚合酶链反应（PCR）检测腺病毒40型和41型。

结果

所采用方法检测到的最常见病毒是粪便中的轮状病毒抗原（35%）。23%的粪便样本腺病毒抗原检测呈阳性，这些样本的PCR检测阳性率为22%。检测到13份样本中存在ADv40，9份样本中存在ADv41。1份腺病毒抗原ELISA检测呈阳性的样本，这些基因型的PCR检测为阴性。ELISA检测到7%的患儿同时感染了轮状病毒和腺病毒。ELISA检测腺病毒抗原呈阳性的患者中，最常见的症状是呕吐（54.5%）和腹痛（45.5%）。与感染其他病原体的患者相比，感染腺病毒的患儿在发热（P = 0.94）和腹痛（P = 0.63）方面无显著差异。在≤24个月的急性胃肠炎患儿中，68.2%检测到腺病毒。与腺病毒检测阴性的患儿（23.1%，P = 0.004，OR 4.0，95%CI：1.5 - 10.8）相比，感染腺病毒的患儿呕吐明显增加。