Tian Na, Yuan Yuan, Jin Yan, Yang Quan, Zhang Tian, Li Jun-de, Wang Li, Jiang Chao, Huang Lu-Qi
College of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700, China.
State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2020 Mar;45(5):1064-1069. doi: 10.19540/j.cnki.cjcmm.20200105.106.
The pig bile powder, bovine bile powder, snake bile, sheep bile, goose bile powder, and bear bile powder were contained by the Chinese Pharmacopoeia. The bile power medicine has a long history in traditional Chinese medicine and definite effect. However, the medicine of bile powder(bile) are similar in morphology. Besides, many medicine lack specific microscopic identification characteristics and chemical characteristics. There is a risk of adulteration, especially when the fake medicine were mixed in authentic medicine, it is difficult to detection. The key to control the quality and ensures the clinical efficacy is the good or bad, true or false of the bile power medicine. The STR typing technology is a method that according to differential typing of PCR amplified lengths to compare and identify individual organisms. Based on the principle of STR typing, the easily, rapid DNA fingerprinting method to identify the bile power and adulteration was established.The original animal or bile powder of pigs, cattle, sheep, chickens, ducks, geese, snakes, bears, fish were collected, the 12 S-L1091/12 S-H1478 and 16 S-L3428/16 S-H3667 was obtained by sifted, the DNA fingerprinting of the bile power and adulteration was obtained by STR typing. Every species has different STR fingerprints, so different species can be identified. Besides, the fingerprints have both the authentic and fake's information, the adulteration of authentic and fake can be identified. Therefore, the method to identify the bile power and adulteration was achieved through the combination of two primers. The DNA fingerprinting method established in this study can also be used for other animal medicine.
《中国药典》收载了猪胆粉、牛胆粉、蛇胆、羊胆、鹅胆粉和熊胆粉。胆粉类药材在中药中应用历史悠久且疗效确切。然而，胆粉类药材形态相似，且许多药材缺乏特异性的显微鉴别特征和化学特征，存在掺伪风险，尤其是假药混入真药中时难以检测。控制胆粉类药材质量、确保临床疗效的关键在于其质量优劣、真伪。STR分型技术是一种根据PCR扩增片段长度差异进行个体生物分型比较鉴定的方法。基于STR分型原理，建立了简便、快速的DNA指纹图谱法用于鉴别胆粉及其掺伪情况。采集猪、牛、羊、鸡、鸭、鹅、蛇、熊、鱼的原动物或胆粉，筛选得到12S-L1091/12S-H1478和16S-L3428/16S-H3667，通过STR分型获得胆粉及其掺伪品的DNA指纹图谱。每个物种都有不同的STR指纹图谱，因此可以鉴别不同物种。此外，指纹图谱包含真品和伪品的信息，能够鉴别真品与伪品的掺伪情况。所以，通过两种引物的组合实现了鉴别胆粉及其掺伪情况的方法。本研究建立的DNA指纹图谱法也可用于其他动物药材。
