Liu Xiao-Yan, Xu Wei, Yang Xiu-Wei, Zhang Peng, Zhao Wei, Gong Yun, Liu Ni-Fu
State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Beijing 100191, China.
Zhuzhou Qianjin Pharmaceutical Co., Ltd. Zhuzhou 412000, China.
Zhongguo Zhong Yao Za Zhi. 2020 Mar;45(5):1120-1127. doi: 10.19540/j.cnki.cjcmm.20200102.201.
To study the non-flavonoids chemical constituents in water extract of Spatholobi Caulis. Some purification and analysis techniques like silica gel, D101-macroporous adsorptive resins, and Sephadex LH-20 column chromatographies as well as reversed phase high-performance liquid chromatography were used to isolate and analyze the phenolic acid esters and other type compounds from Spatholobi Caulis integrally. The structures of these compounds were identified by spectroscopic techniques such as nuclear magnetic resonance and high resolution mass spectrometries. Twenty-seven compounds, including phenolic acid, coumarin, lignan, terpene, alkaloid, and steroid compounds, were isolated from ethyl acetate and n-butanol fractions in water extract of Spatholobi Caulis, and they were identified as β-sitosterol(1), feruli acid methyl ester(2), syringaresinol(3),(+)-medioresinol(4),(+)-epipinoresinol(5), p-acetylphenol(6), bolusanthin Ⅳ(7), evofolin B(8), salicylic acid(9), trans-p-hydroxy-cinnamic acid(10), abscisic acid(11), m-hydroxyphenol(12), C-veratroylglycol(13), p-hydroquinone(14), 8,9-dihydroxymegastigma-4,6-dien-3-one(15), p-hydroxybenzoic acid(16), 6,9-dihydroxymegastigma-4,7-dien-3-one(17), protocatechuic acid(18), protocatechuic acid methyl ester(19), 5,7-dihydroxycoumarin(20), isolariciresinol(21), nicotinic acid(22), daucosterol(23),(+)-pinoresinol(24), stigmasterol(25), allantoin(26) and koaburaside(27), respectively. Furthermore, compounds 2-15, 19-22, 24 and 26 were isolated from genus Spatholobus for the first time.
研究鸡血藤水提取物中的非黄酮类化学成分。采用硅胶、D101大孔吸附树脂、Sephadex LH - 20柱色谱以及反相高效液相色谱等多种分离分析技术，对鸡血藤中的酚酸酯类及其他类型化合物进行整体分离与分析。通过核磁共振、高分辨质谱等波谱技术鉴定这些化合物的结构。从鸡血藤水提取物的乙酸乙酯和正丁醇部位分离得到27个化合物，包括酚酸、香豆素、木脂素、萜类、生物碱及甾体类化合物，它们分别被鉴定为β-谷甾醇(1)、阿魏酸甲酯(2)、紫丁香树脂酚(3)、(+)-中树脂醇(4)、(+)-表松脂醇(5)、对乙酰苯酚(6)、鸡血藤苷Ⅳ(7)、卫矛醇B(8)、水杨酸(9)、反式对羟基肉桂酸(10)、脱落酸(11)、间羟基苯酚(12)、C - 藜芦酰二醇(13)、对苯二酚(14)、8,9 - 二羟基大柱头草-4,6 - 二烯-3 - 酮(15)、对羟基苯甲酸(16)、6,9 - 二羟基大柱头草-4,7 - 二烯-3 - 酮(17)、原儿茶酸(18)、原儿茶酸甲酯(19)、5,7 - 二羟基香豆素(20)、异落叶松脂醇(21)、烟酸(22)、胡萝卜苷(23)、(+)-松脂醇(24)、豆甾醇(25)、尿囊素(26)和柯桠素(27)。此外，化合物2 - 15、19 - 22、24和26为首次从密花豆属植物中分离得到。
