Cao Dong-Min, Guan Qin-Xiao, Liu Ya-Li, Wang Shu-Mei
School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine Guangzhou 510006, China Engineering Technology Research Center for Chinese Materia Medica Quality of Guangdong Province Guangzhou 510006, China.
Zhongguo Zhong Yao Za Zhi. 2020 Mar;45(5):1142-1148. doi: 10.19540/j.cnki.cjcmm.20190619.505.
Serum metabonomic profiles of the model of focal cerebral ischemia reperfusion is established with the suture-occluded method by Longa to study the effect of ginsenosides. In this study, 48 rats were randomly divided into six groups: sham-operated group, pathological model group, positive drug group(6 mg·kg(-1)·d(-1)) and high, medium, low-dose ginsenosides groups(200, 100, 50 mg·kg(-1)·d(-1)). They are given intragastric administration respectively with same amount of 0.5% CMC-Na,nimodipine and ginsenoside for 5 days. At 2 h after the final administration, the model was established with the suture-occluded method, and free radical-scavenging activity changes of ginsenoside were observed by maillard reaction, and Longa was possible used as a renoprotective agent-occluded method. At the end of 24 h after the reperfusion, the hemolymph of rats in each group was collected, and the ~1H-NMR spectrum was collected after being treated by certain methods, and analyzed by principal component analysis(PCA). Compared with sham-operated group, pathological model group showed significant increases in the levels of lactate, glutamate, taurine, choline, glucose and methionine, but decreases in the levels of 3-hydroxybutyrate and phosphocreatine/creatine in serum. After treatment with ginsenosides, lipid, 3-hydroxybutyrate and phosphocreatine/creatine were increased in the serum of ginsenosides group rats, but with decreases in lactate and glutamate. The results showed that ginsenosides could regulate metabolic disorders in rats with focal cerebral ischemia reperfusion, and promote a recovery in the process of metabolism. It's helpful to promote the metabolic changes in rats with focal cerebral ischemia reperfusion via ~1H-NMR, and lay a foundation to develop ginsenosides as a new drug to treat ischemic cerebral paralysis.
采用Longa线栓法建立局灶性脑缺血再灌注模型的血清代谢组学图谱，以研究人参皂苷的作用。本研究将48只大鼠随机分为6组：假手术组、病理模型组、阳性药物组（6mg·kg⁻¹·d⁻¹）和高、中、低剂量人参皂苷组（200、100、50mg·kg⁻¹·d⁻¹）。分别给予等量的0.5%羧甲基纤维素钠、尼莫地平和人参皂苷灌胃5天。末次给药后2小时，采用线栓法建立模型，通过美拉德反应观察人参皂苷清除自由基活性变化，且Longa可能用作肾保护剂阻塞法。再灌注24小时结束时，收集各组大鼠的血淋巴，经一定方法处理后采集¹H-NMR谱，并通过主成分分析（PCA）进行分析。与假手术组相比，病理模型组血清中乳酸、谷氨酸、牛磺酸、胆碱、葡萄糖和蛋氨酸水平显著升高，但3-羟基丁酸和磷酸肌酸/肌酸水平降低。人参皂苷处理后，人参皂苷组大鼠血清中脂质、3-羟基丁酸和磷酸肌酸/肌酸升高，但乳酸和谷氨酸降低。结果表明，人参皂苷可调节局灶性脑缺血再灌注大鼠的代谢紊乱，促进代谢过程的恢复。通过¹H-NMR促进局灶性脑缺血再灌注大鼠的代谢变化，为开发人参皂苷作为治疗缺血性脑瘫的新药奠定基础。
