人参皂苷Rb1对局灶性脑缺血/再灌注损伤模型大鼠脑组织及血清中脑梗死体积和白细胞介素-1β的影响
[Effect of ginsenoside Rb1 on cerebral infarction volume and IL-1 beta in the brain tissue and sera of focal cerebral ischemia/reperfusion injury model rats].
作者信息
Liu Jun-Wei, Ren Ye-Long, Liu Xu-Ling, Xia Hong-Lian, Zhang Hui-Ling, Jin Shen-Hui, Dai Qin-Xue, Wang Jun-Lu
机构信息
Department of Anesthesiology, First Affiliated Hospital, Wenzhou Medical College, Zhejiang 325000, China.
Department of Anesthesiology, Yangquan Coal Group General Hospital, Shanxi 045000, China.
出版信息
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2013 Dec;33(12):1696-700.
OBJECTIVE
To investigate the effect of ginsenoside Rb1 on cerebral infarction volume as well as IL-1 beta in the brain tissue and sera of focal cerebral ischemia/reperfusion (I/R) injury model rats.
METHODS
The I/R rat model was established by using thread according to Zea-Longa. SD rats were randomly divided into five groups, i.e., the sham-operation group, the model group, the low dose ginsenoside Rb1 (20 mg/kg) group, the medium dose ginsenoside Rb1 group (40 mg/kg), and the high dose ginsenoside Rb1 group (80 mg/kg), 12 in each group. Rats in the sham-operation group only received middle cerebral artery occlusion (MCAO) but without thread insertion. The MCAO model was prepared in the rest 4 groups, followed by MCAO2 h later. Ginsenoside Rb1 at each dose was peritoneally administrated to rats in corresponding groups immediately after cerebral ischemia. Equal volume of normal saline was administered to rats in the sham-operation group. Rats' cerebral infarction volume, integrals of neurologic defect degree, expression of IL-1 beta content in the brain tissue and sera were observed 24 h after 2-h cerebral I/R.
RESULTS
In the model group, integrals of neurologic defect degree were improved (P < 0.01), IL-1 beta positive cells in the brain tissue increased and serum IL-1 beta content elevated (P < 0.05), when compared with the sham-operation group. In comparison of the model group, integrals of neurologic defect degree were lowered in the medium dose and high dose ginsenoside Rb1 groups (P < 0.05, P < 0.01). The cerebral infarction volume was all shrunken in each ginsenoside Rb1 group, IL-1 beta positive cells in the brain tissue decreased, and IL-1 beta content in serum reduced (P < 0.01, P < 0.05). Compared with the low dose ginsenoside Rb1 group, integrals of neurologic defect degree decreased, the cerebral infarction volume shrunken, and IL-1 beta content in serum reduced in the high dose ginsenoside Rb1 group (P < 0.01, P < 0.05).
CONCLUSION
Ginsenoside Rb1 (20, 40, 80 mg/kg) might effectively release local cerebral ischemia by down-regulating the IL-1 beta expression.
目的
探讨人参皂苷Rb1对局灶性脑缺血/再灌注（I/R）损伤模型大鼠脑梗死体积以及脑组织和血清中白细胞介素-1β（IL-1β）的影响。
方法
参照Zea-Longa法用线栓法建立I/R大鼠模型。将SD大鼠随机分为5组，即假手术组、模型组、低剂量人参皂苷Rb1（20 mg/kg）组、中剂量人参皂苷Rb1组（40 mg/kg）和高剂量人参皂苷Rb1组（80 mg/kg），每组12只。假手术组大鼠仅行大脑中动脉闭塞（MCAO）但不插入线栓。其余4组制备MCAO模型，2小时后再灌注。脑缺血后立即对相应组大鼠腹腔注射各剂量人参皂苷Rb1。假手术组大鼠注射等体积的生理盐水。在脑I/R 2小时后24小时观察大鼠脑梗死体积、神经功能缺损程度积分、脑组织和血清中IL-1β含量的表达。
结果
与假手术组相比，模型组神经功能缺损程度积分升高（P<0.01），脑组织中IL-1β阳性细胞增多，血清IL-1β含量升高（P<0.05）。与模型组比较，中剂量和高剂量人参皂苷Rb1组神经功能缺损程度积分降低（P<0.05，P<0.01）。各人参皂苷Rb1组脑梗死体积均缩小，脑组织中IL-1β阳性细胞减少，血清中IL-1β含量降低（P<0.01，P<0.05）。与低剂量人参皂苷Rb1组比较，高剂量人参皂苷Rb1组神经功能缺损程度积分降低，脑梗死体积缩小，血清中IL-1β含量降低（P<0.01，P<0.05）。
结论
人参皂苷Rb1（20、40、80 mg/kg）可能通过下调IL-1β表达有效减轻局部脑缺血。
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