Yang Ping, Wang Hong-Lan, Sun Hui, Zhu Wen-Tao, Qiu Tao, DU Jiu-Zhen, Sun Hong-Bing, Zhou Yi, Jiang Shun-Yuan
Sichuan Academy of Chinese Medicine Sciences Chengdu 610041, China.
Department of Environmental Science and Engineering, Sichuan University Chengdu 610065, China.
Zhongguo Zhong Yao Za Zhi. 2020 Feb;45(4):739-745. doi: 10.19540/j.cnki.cjcmm.20191223.103.
Study the growth and development process of rhizomes(bamboo-like part) of Notopterygium incisum and the changes of carbohydrate, endogenous hormones and secondary metabolites, and provide theoretical guidance for the formation of high-quality N. incisum medicinal commodities under artificial cultivation. The One-year-old seedlings were transplanted to the original habitat,and the growth and physiological characteristics of N. incisum were dynamically monitored. The results showed that: ① Seedlings transplanted to the original habitat in spring could form rhizomes(bamboo-like part) in the same year. ② After 60 days of transplantation, the root length and root diameter of underground part of N. incisum had increased rapidly, and carbohydrate content in roots and rhizomes had accumulated rapidly. After 120 days of transplantation, the roots and rhizomes of underground part had grown slowly, and starch content in roots and rhizomes increased continuously, while sucrose and total soluble sugar content decreased gradually. ③ The content of abscisic acid(ABA) in rhizomes decreased firstly and then increased, while the indole acetic acid(IAA) content stabilized firstly and then increased rapidly, and the contents of gibberellin(GA_3) and zeatin riboside(ZR) continued to increase. ④ The content of notopterol in rhizomes was higher than that in roots, while the content of isoimperatorin was lower than that in roots, but the total content of the both in rhizomes was higher than that in roots. Therefore, N. incisum can form rhizomes with high content of secondary metabolites under wild tending, and the growth and development of rhizomes are closely related to changes in carbohydrates and are regulated by related endogenous hormones.
研究羌活根茎（竹节状部分）的生长发育过程以及碳水化合物、内源激素和次生代谢产物的变化，为人工栽培条件下优质羌活药材商品的形成提供理论指导。将一年生幼苗移栽至原生境，动态监测羌活的生长和生理特性。结果表明：①春季移栽至原生境的幼苗当年即可形成根茎（竹节状部分）。②移栽60天后，羌活地下部分根长和根直径迅速增加，根和根茎中碳水化合物含量快速积累。移栽120天后，地下部分根和根茎生长缓慢，根和根茎中淀粉含量持续增加，而蔗糖和总可溶性糖含量逐渐降低。③根茎中脱落酸（ABA）含量先降低后升高，吲哚乙酸（IAA）含量先稳定后迅速升高，赤霉素（GA_3）和玉米素核苷（ZR）含量持续升高。④根茎中羌活醇含量高于根中，异欧前胡素含量低于根中，但二者在根茎中的总含量高于根中。因此，羌活在野生抚育条件下可形成次生代谢产物含量较高的根茎，根茎的生长发育与碳水化合物变化密切相关，并受相关内源激素调控。
