Cold Spring Harb Protoc. 2020 Apr 1;2020(4):099333. doi: 10.1101/pdb.prot099333.
Colloidal gold-antibody conjugates are easy to prepare and are an excellent choice for microscopic applications. Colloidal gold is an aqueous suspension of nanometer-sized particles of gold. Typically, chloroauric acid, HAuCl, is reduced with dilute solutions of sodium citrate, as described here. This will cause the gold to form small aggregates that will associate with proteins. Gold particles of specific sizes can be isolated and differentiated microscopically, allowing these particles to be used for multiple-label experiments. Colloidal gold-labeled antibodies are widely used in electron microscopy (EM), and can be used for light microscopy but require additional steps (silver enhancement).
胶体金-抗体缀合物易于制备，是显微镜应用的绝佳选择。胶体金是纳米金颗粒的水性悬浮液。通常，如这里所述，用柠檬酸三钠的稀溶液还原氯金酸，HAuCl。这将导致金形成小聚集体，与蛋白质结合。可以分离和区分特定大小的金颗粒，从而使这些颗粒可用于多标记实验。胶体金标记的抗体广泛用于电子显微镜（EM），也可用于光学显微镜，但需要额外的步骤（银增强）。
