Institute of Science, Bioengineering Division, Hacettepe University, Ankara, Turkey.
Institue of Science, Nanotechnology and Nanomedicine Division, Hacettepe University, Ankara, Turkey.
Sci Rep. 2020 Apr 1;10(1):5758. doi: 10.1038/s41598-020-62822-1.
There has been substantial interest in research aimed at conductive carbon-based supports since the discovery that the electrical stimulus can have dramatic effect on cell behavior. Among these carbon-aerogels decorated with biocompatible polymers were suggested as future materials for tissue engineering. However, high reaction temperatures required for the synthesis of the aerogels tend to impair the stability of the polymeric networks. Herein, we report a synthetic route towards carbon-aerogel scaffolds decorated with biocompatible ceramic nanoparticles of tricalcium phosphate. The composites can be prepared at temperature as high as 1100 °C without significant effect on the morphology of the composite which is comparable with the original aerogel framework. Although the conductivity of the composites tends to decrease with the increasing ceramic content the measured conductivity values are similar to those previously reported on polymer-functionalized carbon-aerogels. The cell culture study revealed that the developed constructs support cell proliferation and provide good cell attachment suggesting them as potentially good candidates for tissue-engineering applications.
自发现电刺激对细胞行为有显著影响以来,人们对导电碳基载体的研究产生了浓厚的兴趣。在这些经过生物相容性聚合物修饰的碳气凝胶中,它们被认为是未来组织工程的材料。然而,气凝胶合成所需的高反应温度往往会破坏聚合物网络的稳定性。在此,我们报告了一种通过生物相容性陶瓷磷酸三钙纳米粒子来修饰碳气凝胶支架的合成途径。该复合材料可以在高达 1100°C 的温度下制备,而不会对复合材料的形态产生显著影响,其与原始气凝胶骨架相当。尽管复合材料的电导率随着陶瓷含量的增加而降低,但测量的电导率值与先前报道的聚合物功能化碳气凝胶相似。细胞培养研究表明,所开发的结构支持细胞增殖,并提供良好的细胞附着,表明它们可能是组织工程应用的良好候选材料。