大鼠肝微粒体细胞色素b5。一种通过含抗原脂质体进行简单大规模纯化及抗体生产的方法。
Rat hepatic microsomal cytochrome b5. A simple large-scale purification procedure and antibody production by antigen-containing liposomes.
作者信息
Carlsen J, Christiansen K, Jensen H M
机构信息
Department of Biochemistry C, Panum Institute, University of Copenhagen, Denmark.
出版信息
Biochem J. 1988 Dec 15;256(3):1051-4. doi: 10.1042/bj2561051.
Abstract
Cytochrome b5 from rat liver microsomes (microsomal fractions) was purified in its native form. The procedure described has great capacity, is fast, and the final product is pure as judged from SDS/polyacrylamide-gel electrophoresis. Antibodies to cytochrome b5 are obtained after administration of the antigen inserted into small unilamellar lipid vesicles.
摘要
从大鼠肝脏微粒体（微粒体部分）中以天然形式纯化出细胞色素b5。所描述的方法容量大、速度快，并且从SDS/聚丙烯酰胺凝胶电泳判断，最终产物是纯的。将抗原插入小单层脂质囊泡后进行给药，从而获得针对细胞色素b5的抗体。
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