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植物细胞色素b5的纯化及性质

Purification and properties of plant cytochrome b5.

作者信息

Bonnerot C, Galle A M, Jolliot A, Kader J C

出版信息

Biochem J. 1985 Feb 15;226(1):331-4. doi: 10.1042/bj2260331.

DOI:10.1042/bj2260331
PMID:3977878
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1144710/
Abstract

Microsomal cytochrome b5 was 352-fold purified from potato tubers with a yield of 10.4%. To our knowledge, this is the first report relating the purification of higher-plant cytochrome b5. Its Mr (16 700) and absorption spectrum are similar to those of animal and yeast cytochrome b5.

摘要

微粒体细胞色素b5从马铃薯块茎中纯化了352倍，产率为10.4%。据我们所知，这是关于高等植物细胞色素b5纯化的首次报道。其相对分子质量（16700）和吸收光谱与动物及酵母细胞色素b5的相似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7b8/1144710/e39a1933f35b/biochemj00309-0323-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7b8/1144710/e39a1933f35b/biochemj00309-0323-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7b8/1144710/e39a1933f35b/biochemj00309-0323-a.jpg

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本文引用的文献

1
A nondenaturing zwitterionic detergent for membrane biochemistry: design and synthesis.一种用于膜生物化学的非变性两性离子去污剂：设计与合成。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6368-70. doi: 10.1073/pnas.77.11.6368.
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Isolation and characterization of cytochrome b5 from Candida tropicalis grown on alkane.从在烷烃上生长的热带假丝酵母中分离并鉴定细胞色素b5
Biochimie. 1982 Nov-Dec;64(11-12):1041-8. doi: 10.1016/s0300-9084(82)80385-4.
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Purification of a NADH-ferricyanide reductase from plant microsomal membranes with a zwitterionic detergent.
从花椰菜（ Brassica oleracea L.）的肽和 cDNA 序列推导出细胞色素 b(5)的一级结构。
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Purification and Characterization of Microsomal Cytochrome b(5) and NADH Cytochrome b(5) Reductase from Pisum sativum.豌豆微粒体细胞色素 b(5)和 NADH 细胞色素 b(5)还原酶的纯化和特性。
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Temporal and spatial gene expression of cytochrome B5 during flower and fruit development in olives.橄榄花和果实发育过程中细胞色素B5的时空基因表达
Plant Mol Biol. 1999 May;40(1):79-90. doi: 10.1023/a:1026417710320.
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Microsomal electron transfer in higher plants: cloning and heterologous expression of NADH-cytochrome b5 reductase from Arabidopsis.高等植物中的微粒体电子传递：拟南芥NADH-细胞色素b5还原酶的克隆与异源表达
Plant Physiol. 1999 Jan;119(1):353-62. doi: 10.1104/pp.119.1.353.
7
Electron-transport components of the 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine delta 12-desaturase (delta 12-desaturase) in microsomal preparations from developing safflower (Carthamus tinctorius L.) cotyledons.来自发育中的红花（Carthamus tinctorius L.）子叶微粒体制剂中1-酰基-2-油酰基-sn-甘油-3-磷酸胆碱δ12-去饱和酶（δ12-去饱和酶）的电子传递成分。
Biochem J. 1990 Nov 15;272(1):23-9. doi: 10.1042/bj2720023.
用两性离子去污剂从植物微粒体膜中纯化NADH-铁氰化物还原酶。
Biochem Biophys Res Commun. 1984 Aug 16;122(3):1201-5. doi: 10.1016/0006-291x(84)91219-1.
4
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A form of cytochrome b5 that contains an additional hydrophobic sequence of 40 amino acid residues.一种细胞色素b5，其包含一个额外的由40个氨基酸残基组成的疏水序列。
Proc Natl Acad Sci U S A. 1971 May;68(5):1042-6. doi: 10.1073/pnas.68.5.1042.
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Proc Natl Acad Sci U S A. 1974 Nov;71(11):4565-9. doi: 10.1073/pnas.71.11.4565.
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Cytochrome components of plant microsomes.植物微粒体的细胞色素成分。
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
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