一种纯化大鼠肝微粒体细胞色素b5的简单方法。
A simple method for purification of rat hepatic microsomal cytochrome b5.
作者信息
Burchell A
出版信息
Biochem J. 1985 Feb 15;226(1):339-41. doi: 10.1042/bj2260339.
Abstract
Rat hepatic microsomal cytochrome b5 was purified to homogeneity by solubilization with the detergent Lubrol 12-A9 and chromatography on Fractogel TSK DEAE-650(S). The protein was obtained in high yield (52-87%) in 8 h, and only one polypeptide band, of Mr 16 600, was visible after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.
摘要
通过用去污剂Lubrol 12-A9增溶并在Fractogel TSK DEAE-650(S)上进行色谱分离，将大鼠肝微粒体细胞色素b5纯化至同质。该蛋白质在8小时内以高产率（52-87%）获得，在十二烷基硫酸钠/聚丙烯酰胺凝胶电泳后仅可见一条分子量为16600的多肽带。
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