Meyer T, Koch R, Fanick W, Hilz H
Institut für Physiologische Chemie, Universität Hamburg, Germany.
Biol Chem Hoppe Seyler. 1988 Jul;369(7):579-83. doi: 10.1515/bchm3.1988.369.2.579.
Various types of ADP-ribosyl protein conjugates were synthesized and their chemical stability was compared with that of cysteine-linked ADP-ribosyl groups as formed by incubation of transducin or Gi/Go proteins with NAD and pertussis toxin. Treatment with 0.1 mM HgCl2 specifically cleaved the cysteine-linked conjugates. This may provide a tool for the quantitation of modified Gi/Go proteins as well as of other acceptors modified by ADP-ribose at cysteine residues in the presence of other ADP-ribosyl proteins.
合成了各种类型的ADP-核糖基化蛋白质缀合物，并将其化学稳定性与通过转导素或Gi/Go蛋白与NAD和百日咳毒素孵育形成的半胱氨酸连接的ADP-核糖基团的化学稳定性进行了比较。用0.1 mM HgCl2处理可特异性切割半胱氨酸连接的缀合物。这可能为在存在其他ADP-核糖基化蛋白质的情况下，定量修饰的Gi/Go蛋白以及在半胱氨酸残基处被ADP-核糖修饰的其他受体提供一种工具。
