College of Life Science and Technology, Xinjiang University, Urumqi, China.
College of Life Sciences, Linyi University, Linyi, China.
PLoS One. 2020 Apr 2;15(4):e0230924. doi: 10.1371/journal.pone.0230924. eCollection 2020.
The endophytic bacterial diversity of rose was analyzed by high-throughput sequencing of 16S rDNA and functional prediction of the bacterial community. The number of bacterial sequence reads obtained from 18 rose samples ranged from 63,951 to 114,833, and reads were allocated to 1982 OTUs based on sequences of the V3-V4 region. The highest Shannon Index was found in Luogang rose (1.93), while the lowest was found in Grasse rose (0.35). The bacterial sequence reads were grouped into three different phyla: Firmicutes, Proteobacteria, and Actinobacteria. At the genus level, Bacillus and Staphylococcus had the highest abundance across all 18 samples; Bacillus was particularly abundant in Daguo rose (99.09%), Rosa damascena (99.65%), and Fenghua rose (99.58%). Unclassified OTUs were also found in all samples. PICRUSt gene prediction revealed that each endophyte sample contained multiple KEGG functional modules related to human metabolism and health. A high abundance of functional genes were involved in (1) Amino Acid Metabolism, (2) Carbohydrate Metabolism, (3) Cellular Processes and Signaling, (4) Energy Metabolism, and (5) Membrane Transport, indicating that the endophytic community comprised a wide variety of microorganisms and genes that could be used for further studies. The rose endophytic bacterial community is rich in diversity; community composition varies among roses and contains functional information related to human health.
采用高通量测序 16S rDNA 分析了玫瑰内生细菌的多样性，并对细菌群落进行了功能预测。从 18 个玫瑰样本中获得的细菌序列读数数量范围为 63951 到 114833，根据 V3-V4 区域的序列将读数分配到 1982 个 OTUs。洛岗玫瑰的 Shannon 指数最高（1.93），而格拉斯玫瑰的 Shannon 指数最低（0.35）。细菌序列读数分为三个不同的门：厚壁菌门、变形菌门和放线菌门。在属水平上，芽孢杆菌属和葡萄球菌属在所有 18 个样本中丰度最高；芽孢杆菌属在大国玫瑰（99.09%）、大马士革玫瑰（99.65%）和奉化玫瑰（99.58%）中特别丰富。未分类的 OTUs 也存在于所有样本中。PICRUSt 基因预测显示，每个内生样本都包含多个与人类代谢和健康相关的 KEGG 功能模块。大量功能基因参与（1）氨基酸代谢、（2）碳水化合物代谢、（3）细胞过程和信号转导、（4）能量代谢和（5）膜转运，表明内生群落包含了广泛的微生物和基因，可用于进一步研究。玫瑰内生细菌群落丰富多样；群落组成在不同的玫瑰之间存在差异，包含与人类健康相关的功能信息。
