Instituto de Investigaciones en Ecosistemas y Sustentabilidad, Universidad Nacional Autónoma de México, Morelia, Michoacán, Mexico.
Laboratorio Nacional de Análisis y Síntesis Ecológica (LANASE), Escuela Nacional de Estudios Superiores Unidad Morelia, Universidad Nacional Autónoma de México, Morelia, Michoacán, México.
Mol Biol Rep. 2020 May;47(5):4003-4007. doi: 10.1007/s11033-020-05397-4. Epub 2020 Apr 2.
Microsatellite markers are a useful genetic tool to answer ecological and conservation genetics questions. Microsatellite primers were developed and characterized to evaluate forest fragmentation effects on genetic structure, diversity and gene flow patterns in the dioecious tropical tree Astroniumgraveolens (Anacardiaceae). Using genomic library enrichment, sixteen microsatellite loci were developed for A.graveolens. Polymorphism was evaluated in 80 individuals from four localities in Mexico. The overall number of alleles detected was 63, average alleles per locus 3.9 with a range from one to 11 per locus. Cross amplification trails on related species in the Anacardiaceae family: Spondiaspurpurea and Amphipterygiumadstringens, achieved successful amplification for all primers. Microsatellite markers described here are the first to be characterized for A.graveolens. These genetic markers will be a useful tool to assess the genetic consequences of habitat fragmentation and selective logging on A.graveolens populations. Cross amplification success on S.purpureaandA.adstringens suggest that they may be used for population genetic studies in other species within the family.
微卫星标记是一种有用的遗传工具，可以回答生态和保护遗传学问题。为了评估森林破碎化对雌雄异株热带树种 Astroniumgraveolens（漆树科）遗传结构、多样性和基因流模式的影响，我们开发并鉴定了微卫星引物。利用基因组文库富集技术，为 A.graveolens 开发了 16 个微卫星位点。在来自墨西哥四个地点的 80 个个体中评估了多态性。共检测到 63 个等位基因，每个位点的平均等位基因数为 3.9，范围从每个位点 1 到 11。对漆树科相关物种 Spondiaspurpurea 和 Amphipterygiumadstringens 的交叉扩增试验均成功扩增了所有引物。本文描述的微卫星标记是首次对 A.graveolens 进行的特征描述。这些遗传标记将是评估生境破碎化和选择性采伐对 A.graveolens 种群遗传影响的有用工具。在 S.purpurea 和 A.adstringens 上的交叉扩增成功表明，它们可能用于该科内其他物种的种群遗传研究。
