Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St Paul, MN, 55108, USA.
Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St Paul, MN, 55108, USA.
Exp Cell Res. 2020 May 15;390(2):111986. doi: 10.1016/j.yexcr.2020.111986. Epub 2020 Mar 30.
The lack of available, well characterized, established, domestic porcine cell lines hinders the advancement of porcine cellular immunology. A case of multicentric lymphoma was diagnosed in a market weight pig at the time of slaughter. Affected lymph nodes and spleen were collected and used for single cell isolation and analysis. Cell lines were established by 3 rounds of limiting dilution from splenic and subiliac lymph node lymphomas. Surface marker staining identified the cells as CD21, CD79a, CD20, PAX5, and CD3 and cells were grown and easily passaged in cell culture. Transcriptome analysis was carried out to further characterize these rapidly proliferating cells validating the initial cytometric findings, confirming their identity as B cell lymphomas, and suggesting that they arose from germinal center centroblasts with aberrant control of BCL6 expression. Functional analysis identified the cells as being involved in cancer, cell movement, cell survival, and apoptosis. These new porcine B cell lymphoma cell lines will be a valuable resource for more in-depth cellular investigations into the porcine immune system and cancer, as well as providing a potential tool for the growth of lymphotropic viruses of pigs and humans.
缺乏可用的、特征良好的、已建立的、国内的猪细胞系阻碍了猪细胞免疫学的发展。在屠宰时，一头商品重猪被诊断出患有多中心淋巴瘤。收集受影响的淋巴结和脾脏，用于单细胞分离和分析。通过脾和髂下淋巴结淋巴瘤的 3 轮有限稀释建立了细胞系。表面标记染色将细胞鉴定为 CD21、CD79a、CD20、PAX5 和 CD3，细胞在细胞培养中生长并易于传代。进行转录组分析以进一步表征这些快速增殖的细胞，验证初始细胞计数的发现，确认它们是 B 细胞淋巴瘤，并表明它们起源于生发中心中心母细胞，BCL6 表达异常控制。功能分析表明这些细胞参与癌症、细胞运动、细胞存活和细胞凋亡。这些新的猪 B 细胞淋巴瘤细胞系将成为深入研究猪免疫系统和癌症的宝贵资源，并为猪和人类的淋巴病毒生长提供潜在工具。
