壳聚糖包裹的硫纳米粒子的抗菌和抗真菌活性比较。

Comparative antibacterial and antifungal activities of sulfur nanoparticles capped with chitosan.

机构信息

Department of Food and Nutrition, BioNanocomposite Research Center, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea.

出版信息

Microb Pathog. 2020 Jul;144:104178. doi: 10.1016/j.micpath.2020.104178. Epub 2020 Mar 30.

DOI:10.1016/j.micpath.2020.104178

PMID:32240768

Abstract

The antimicrobial activity of sulfur nanoparticles (SNPs) was compared with elemental sulfur and sulfur-containing salts (sodium thiosulfate and sodium metabisulfite) against bacteria (Escherichia coli, Staphylococcus aureus) and fungi (Aspergillus flavus, Candida albicans) using the paper disc, broth microdilution, and time-kill assay methods. The results of the paper disc and MIC tests showed stronger antimicrobial activity of SNPs compared to the elemental sulfur and sulfur-containing salts. SNPs showed more potent activity against bacteria than fungi. Among the test microorganisms, E. coli (Gram-negative) was the most susceptible to SNPs, followed by S. aureus (Gram-positive), C. albicans (yeast), and A. flavus (mold). Scanning electron micrographs of microorganisms treated with SNPs showed different cell disruption patterns depending on the type of microorganisms.

摘要

采用纸片扩散法、微量肉汤稀释法和时间杀菌法，比较了硫纳米粒子（SNPs）与元素硫和含硫盐（硫代硫酸钠和亚硫酸氢钠）对细菌（大肠杆菌、金黄色葡萄球菌）和真菌（黄曲霉、白色念珠菌）的抗菌活性。纸片扩散法和 MIC 试验结果表明，SNPs 的抗菌活性明显强于元素硫和含硫盐。SNPs 对细菌的活性强于真菌。在所测试的微生物中，革兰氏阴性菌大肠杆菌对 SNPs 最敏感，其次是革兰氏阳性菌金黄色葡萄球菌、酵母白色念珠菌和霉菌黄曲霉。用 SNPs 处理后的微生物的扫描电子显微镜照片显示，根据微生物的类型，细胞破裂模式不同。