Departamento de Química Analítica, Instituto Universitario de Investigación en Química Fina y Nanoquímica IUIQFN, Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie Anexo, E-14071 Córdoba, Spain.
Nanobiology Laboratory, Department of Natural and Exact Sciences, Pontificia Universidad Católica Madre y Maestra, PUCMM, Santiago de los Caballeros, Dominican Republic.
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109805. doi: 10.1016/j.msec.2019.109805. Epub 2019 May 28.
In recent years, both nanotechnology and the use of nanomaterials have been growing in fields as diverse as biomedicine, food or electronics. Particularly metal nanoparticles, such as gold nanoparticles (AuNPs), are being widely studied with different applications, for example as an antimicrobial agent, in hyperthermic therapy or drug transport. Gold nanoparticles can be synthesized by different methods, such as stand out reduction with citrate or greener methods that use greener reducing agents (e.g. stainless steel). In the present work, both the effect of the synthesis method yielding AuNPs with similar size and purification of AuNPs affect the antibacterial and antifungal activities of the AuNPs obtained by citrate reduction and with stainless steel. The growth curves of the gram-negative (E. coli) and gram-positive bacteria (S. aureus) and the yeast C. albicans were constructed and the cell viability was evaluated by (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT). According to our results, the purification of the AuNPs after their synthesis and the growth determination method affect the antibacterial and antifungal activities while the synthesis method shows no significant differences.
近年来，纳米技术和纳米材料的应用在生物医学、食品和电子等领域得到了迅速发展。特别是金属纳米粒子，如金纳米粒子（AuNPs），因其具有抗菌剂、热疗或药物输送等不同的应用而受到广泛研究。金纳米粒子可以通过不同的方法合成，例如使用柠檬酸进行标准还原或使用更环保的还原剂（如不锈钢）进行更环保的还原方法。在本工作中，合成方法的效果以及 AuNPs 的纯化都会影响柠檬酸还原法和不锈钢还原法得到的 AuNPs 的抗菌和抗真菌活性。构建了革兰氏阴性（E. coli）和革兰氏阳性菌（S. aureus）以及酵母 C. albicans 的生长曲线，并通过（2,3-双-（2-甲氧基-4-硝基-5-磺苯基）-2H-四唑-5-羧基苯胺（XTT）评估细胞活力。根据我们的结果，AuNPs 合成后的纯化和生长测定方法会影响其抗菌和抗真菌活性，而合成方法则没有显示出显著的差异。
