State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 211198, People's Republic of China.
State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 211198, People's Republic of China.
Bioorg Chem. 2020 Jun;99:103795. doi: 10.1016/j.bioorg.2020.103795. Epub 2020 Mar 24.
Eight new alkaloids, including five isoquinoline alkaloids, a benzoazepine alkaloid, two isoindole alkaloids, and three synthetic alkaloids firstly obtained from the natural sources, together with three known ones were isolated from the bulbs of Corydalis decumbens. The structures were determined by analysis of their spectroscopic data and single-crystal X-ray diffraction. This is the first report of isoindole alkaloid and benzoazepine alkaloid from the genus Corydalis. Full NMR data for 9-11 are reported here for the first time. Moreover, the ability to modulate neuronal Ca mobilization of the isolated alkaloids was tested in primary cultured neocortical neurons. Compound 7 inhibited spontaneous Ca oscillations in primary neocortical neuron cultures at low micromolar concentrations.
从延胡索属植物中首次分离得到 8 种新的生物碱，包括 5 种异喹啉生物碱、1 种苯并氮杂卓生物碱、2 种异吲哚生物碱和 3 种合成生物碱，此外还分离得到 3 种已知生物碱。通过分析其光谱数据和单晶 X 射线衍射确定了它们的结构。这是首次从延胡索属植物中分离得到异吲哚生物碱和苯并氮杂卓生物碱。本文首次报道了 9-11 的全 NMR 数据。此外，还测试了分离得到的生物碱调节原代培养皮质神经元钙动员的能力。在低微摩尔浓度下，化合物 7 抑制原代皮质神经元培养中的自发钙振荡。
