School of Pharmacy, Devi Ahilya University, Indore, 452017, India.
School of Life Sciences, Devi Ahilya University, Indore, 452017, India.
Mol Biol Rep. 2020 Apr;47(4):2801-2810. doi: 10.1007/s11033-020-05405-7. Epub 2020 Apr 3.
Untreated hyperthyroidism may develop serious complications. This attempt was made to investigate the potential of Aloe vera gel in regulating experimentally induced hyperthyroidism in rats. Female Wistar rats were made hyperthyroid with L-thyroxine (L-T) at 0.5 mg/kg/day, i.p. for 14 days and the effects of Aloe vera methanolic fraction (AVMF) (50 or 500 mg/kg/day, p.o.,) and a conventional antithyroid drug propylthiouracil (PTU) (10 mg/kg, i.p.) for 30 days were studied in those hyperthyroid rats. At the end, alterations in serum thyroid hormones and thyroid stimulating hormone (TSH); hepatic 5'mono-deiodinase-1(5'D1) activity, oxidative stress markers and antioxidants; serum inflammatory cytokines and the expression of thyrotropin receptor in thyroid gland were evaluated in all experimental animals. Hyperthyroid condition was confirmed by an increase in thyroid hormone levels and hepatic 5'D-1 activity with a decrease in TSH. However, either AVMF or PTU treatment in hyperthyroid rats decreased the levels of thyroid hormones and 5'D1 activity. AVMF administration in T-induced rats also decreased the oxidative stress markers such as thiobarbituric acid reactive substances and lipid hydroperoxides and increased the antioxidant levels in liver tissues. Levels of liver marker enzymes, cytokines and different lipids were decreased in T-induced AVMF treated rats. Further, a down regulation in the TSHR expression in thyroid was observed in AVMF or PTU treated groups. All these thyroid inhibiting effects were supported by an improvement in thyroid histology in hyperthyroid rats. It appears, about 15 compounds, as evidenced by LC-MS/MS study, mostly phenolics are involved in this anti-thyroid effects of the test compound.
未经治疗的甲状腺功能亢进症可能会引发严重的并发症。本研究旨在探讨库拉索芦荟凝胶在调节实验性大鼠甲状腺功能亢进症中的潜在作用。雌性 Wistar 大鼠通过腹腔注射 L-甲状腺素（L-T）（0.5mg/kg/天），连续 14 天建立甲状腺功能亢进症模型，然后用库拉索芦荟甲醇提取物（AVMF）（50 或 500mg/kg/天，灌胃）和传统抗甲状腺药物丙硫氧嘧啶（PTU）（10mg/kg，腹腔注射）治疗 30 天，观察其对甲状腺功能亢进大鼠的影响。最后，评估了所有实验动物的血清甲状腺激素和促甲状腺激素（TSH）变化；肝 5'单脱碘酶-1（5'D1）活性、氧化应激标志物和抗氧化剂；血清炎症细胞因子和甲状腺中的促甲状腺激素受体表达。甲状腺激素水平和肝 5'D-1 活性升高，TSH 降低，证实了甲状腺功能亢进状态。然而，AVMF 或 PTU 治疗甲状腺功能亢进大鼠均可降低甲状腺激素和 5'D1 活性。AVMF 还可降低 T 诱导大鼠的氧化应激标志物，如硫代巴比妥酸反应物和脂质过氧化物，并增加肝组织中的抗氧化剂水平。T 诱导的 AVMF 治疗大鼠的肝标志物酶、细胞因子和不同脂质水平降低。此外，AVMF 或 PTU 治疗组甲状腺中的 TSHR 表达下调。所有这些甲状腺抑制作用均得到甲状腺组织学改善的支持。通过 LC-MS/MS 研究表明，大约有 15 种化合物，主要是酚类，参与了该测试化合物的抗甲状腺作用。
