Rongala Suma, Kolusu Aravinda Sai, Jakkamsetti Madhuri Suma, Mohanty Sujit Kumar, Samudrala Pavan Kumar, Arakareddy Bhanu Prakash
Department of Pharmacology, Shri Vishnu College of Pharmacy (SVCP) - Vishnupur, West Godavari, Bhimavaram, 534202, Andhra Pradesh, India.
Department of Pharmaceutical Chemistry, Shri Vishnu College of Pharmacy (SVCP) - Vishnupur, West Godavari, Bhimavaram, 534202, Andhra Pradesh, India.
Endocrine. 2024 Oct;86(1):215-232. doi: 10.1007/s12020-024-03818-z. Epub 2024 Apr 18.
Hypothyroidism is an endocrine disorder characterised by decreased T3, T4 and increased TSH levels. This study aims to examine the potential effects of Ferulic acid (FA) on rats with hypothyroidism induced by propylthiouracil through the estimation of biochemical parameters and histopathological studies.
Twenty-five female wistar rats were allocated into five groups: Control group [1% CMC, p.o.], Disease group [PTU-50 mg/kg, p.o.], [Levothyroxine (LT4) group - 20 µg/kg, p.o. + PTU-50 mg/kg, p.o.], [FA -25 mg/kg, p.o. + PTU-50 mg/kg, p.o.] and [FA 50 mg/kg, p.o. + PTU-50 mg/kg, p.o.]. On 15th day blood was collected and serum was separated for estimation of biochemical parameters, liver and kidney homogenate was utilised for the estimation of oxidative stress markers and the thyroid gland was dissected to examine histological features.
PTU administration for 14 days showed a substantial decline in T3 and T4 and increases in TSH levels. PTU-administered rats significantly increased TC, TG and LDL levels, and decreased HDL levels. AST, ALT, urea, creatinine, and IL-6 were determined and these levels were significantly altered in PTU-induced hypothyroid group. In hypothyroid rats MDA, NO, GSH and SOD levels were significantly altered. However, treatment with FA for 14 days attenuated PTU-induced alterations. Furthermore, FA improves the histological changes of the thyroid gland.
In conclusion, FA treatment showed a protective effect against hypothyroidism by stimulating the thyroid hormones through the activation of thyroid peroxidase enzyme and improving thyroid function. In addition, FA diminished the increase in lipids, liver and kidney markers, oxidative stress and inflammation.
甲状腺功能减退症是一种内分泌紊乱疾病，其特征为三碘甲状腺原氨酸（T3）、甲状腺素（T4）水平降低以及促甲状腺激素（TSH）水平升高。本研究旨在通过评估生化参数和组织病理学研究，探讨阿魏酸（FA）对丙硫氧嘧啶诱导的甲状腺功能减退大鼠的潜在影响。
将25只雌性Wistar大鼠分为五组：对照组[口服1%羧甲基纤维素（CMC）]、疾病组[口服丙硫氧嘧啶（PTU）-50mg/kg]、[左甲状腺素（LT4）组-口服20μg/kg+口服PTU-50mg/kg]、[FA-25mg/kg，口服+口服PTU-50mg/kg]和[FA 50mg/kg，口服+口服PTU-50mg/kg]。在第15天采集血液并分离血清以评估生化参数，利用肝脏和肾脏匀浆评估氧化应激标志物，解剖甲状腺以检查组织学特征。
给予PTU 14天显示T3和T4显著下降，TSH水平升高。给予PTU的大鼠总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白（LDL）水平显著升高，高密度脂蛋白（HDL）水平降低。测定了天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、尿素、肌酐和白细胞介素-6（IL-6），这些水平在PTU诱导的甲状腺功能减退组中显著改变。在甲状腺功能减退大鼠中，丙二醛（MDA）、一氧化氮（NO）、谷胱甘肽（GSH）和超氧化物歧化酶（SOD）水平显著改变。然而，给予FA 14天可减轻PTU诱导的改变。此外，FA改善了甲状腺的组织学变化。
总之，FA治疗通过激活甲状腺过氧化物酶刺激甲状腺激素并改善甲状腺功能，对甲状腺功能减退症具有保护作用。此外，FA减少了脂质、肝脏和肾脏标志物的增加、氧化应激和炎症。
