Molecular Biology and Genetics Department, Faculty of Arts and Sciences, Uşak University, 1 Eylül Campus, 64300, Uşak, Turkey.
Environ Sci Pollut Res Int. 2020 Jun;27(16):20335-20343. doi: 10.1007/s11356-020-08553-2. Epub 2020 Apr 2.
Imazalil (IMZ), a fungicide containing imidazole group, is extensively used for the prevention and treatment of fungal diseases in plants. Current study was performed to examine cyto-genotoxic potential of IMZ on Allium cepa roots by following Allium ana-telophase and single cell gel electrophoresis (comet) assays. The concentration which reduced the growth of the root tips of IMZ by 50% compared to the negative control group (EC) was found to be 1 μg/mL by Allium root growth inhibition test. 0.5, 1, and 2 μg/mL concentrations of IMZ were exposed to Allium roots for intervals of 24, 48, 72, and 96 h. 10 μg/mL of methyl methane sulfonate (MMS) and distilled water were used as control groups, both positive and negative. Statistical analysis was performed by using one-way ANOVA with Duncan's multiple comparison tests at p ≤ 0.05 and Pearson correlation test at p = 0.01. IMZ showed cytotoxic effect by statistically decreasing root growth and mitotic index (MI) and also genotoxic effect by statistically increasing chromosomal aberrations (CAs) and DNA damage compared to the negative control group. With these cyto-genotoxic effects, it should be used carefully and further cyto-genotoxic mechanisms should be investigated along with other toxicity tests.
抑霉唑(IMZ)是一种含咪唑基团的杀菌剂,广泛用于预防和治疗植物真菌病。本研究采用洋葱根尖细胞有丝分裂末期观察和单细胞凝胶电泳(彗星)试验,检测 IMZ 对洋葱根尖的细胞遗传毒性。通过洋葱根尖生长抑制试验发现,与阴性对照组相比,能使根尖生长减少 50%的 IMZ 浓度为 1μg/mL。将 0.5、1 和 2μg/mL 的 IMZ 浓度分别暴露于洋葱根尖 24、48、72 和 96 小时。10μg/mL 的甲磺酸甲酯(MMS)和蒸馏水分别作为阳性和阴性对照组。采用单因素方差分析(ANOVA)和 Duncan 多重比较检验(p≤0.05)及 Pearson 相关检验(p=0.01)进行统计学分析。与阴性对照组相比,IMZ 表现出细胞毒性作用,可显著降低根生长和有丝分裂指数(MI);同时也表现出遗传毒性作用,可显著增加染色体畸变(CAs)和 DNA 损伤。鉴于其细胞遗传毒性作用,IMZ 的使用应谨慎,并应结合其他毒性试验进一步研究其细胞遗传毒性机制。